introduction: measles is a severe contagious disease preventable by vaccination. besides the prevalence of measles in iran, ministry of health conducted a nation-wide vaccination against measles for all the 5-25 years old population in 2003. this study was done to evaluate the anti measles igg antibody among the medical students from baqiyatallah university of medical science. materials & metho...

background: during the last twenty years, the extraction of specific egg yolk (igy) antibodies from the immunized chickens has been accepted as a useful alternative to the immunization of mammals. the aim of the present study was immunizing the chickens with human umbilical cord serum (hucs) and the extraction of specific anti-human globulins (igg, c3b, and c3d) antibodies from egg yolk in orde...

background: recombinant monoclonal antibodies have been marketed in last three decades as the major therapeutic proteins against different cancers. however choosing a proper medium and supplements to reach the high expression is a challenging step. despite of commercial serum free and chemically defined media, there are still numerous researches seeking the optimum media to gain higher expressi...

results based on our results, mab against recombinant erns was produced and reacted successfully with recombinant and natural antigens. conclusions with regards to the role of erns in the identification of pi animals, it appears that erns recombinant antigen and the specific monoclonal antibodies produced against it may be suitable for developing bvdv laboratory diagnostic assays. objectives er...

background: the affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as elisa. affinity of most igg specific mabs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...

background: invariant natural killer cells (inkt) are an important immunoregulatory t cell subset. currently several flow cytometry-based approaches exist for the identifi-cation of inkt cells, which rely on using the 6b11 monoclonal antibody or a combina-tion of anti-vα24 and anti-vβ11 antibodies. objective: the aim of this study was to compare the ability of two flow cytometry-based methods f...

background: myocardial infarction (mi) is one of the most common and serious diseases resulting from coronary artery occlusion and major reduction in blood flow. streptokinase as a thrombolytic is considered the first and most important therapeutic intervention for reperfusion following mi in most countries including iran. our previous study showed that, the prevalence of high antibody titers a...

in order to determine the role of different anti-phospholipid antibodies as an etiologic factor in recurrent pregnancy failure, a prospective study was done on one-hundred and thirty-eight women who had unexplained recurrent pregnancy loss (group i) with one-hundred well-matched women with normal reproductive outcome allocated as control group (gil). sera from 138 patients and 100 controls were...

abstract background a vast variety of factors may cause recurrent pregnancy loss. blood group incompatibility of parents could cause abortion. the examination of couples or twins, blood groups showed that the blood group incompatibility can affect adversely the outcome of pregnancy. couples with blood group incompatibility are more involved in spontaneous miscarriage. antigenes in two different...

A novel lateral flow immunoassay biosensor, combining the receptor-ligand reaction and antigen-antibody was developed for detection of ?-lactam antibiotics in milk. The receptor protein, carboxy-terminal domain sensor-transducer mutant from Bacillus. licheniformis, served as recognition element. Colloidal gold (CG)-labelled antibody-receptor ternary complex applied a signal transducer probe. pr...