background: discovery of short cell free fetal dna (cffdna) fragments in maternal plasma has created major changes in the field of prenatal diagnosis. the use of cffdna to set up noninvasive prenatal test is limited due to the low concentration of fetal dna in maternal plasma therefore, employing a high efficiency extraction method leads to more accurate results. the aim of this study was to ev...

background: group  b  streptococcus   (gbs)  is  one  of  the  most  important  cause  of  morbidity  and  mortality  among  newborns  especially  in  developing  countries.  it  has  been  shown  that  the  screening  approach  rather  than  the  identification  of  maternal  clinical  risk  factors  for  early-onset  neonatal  gbs  disease  is  more  effective  in  preventing  early-onset  gb...

background: escherichia coli is a commensal-pathogenic organism, which includes a wide range of strains. despite several advanced molecular-genomic technologies for detecting and identifying different strains of e. coli, enterobacterial repetitive intergenic consensus polymerase chain reaction (eric-pcr) technique is a quick, sharp and cost effective fingerprint method. the major purpose of the...

conclusions results showed that pif that is being consumed in iran is contaminated with this pathogen and can cause disease in infants; especially those hospitalized in neonatal intensive care units (nicus) and fed pif. the number of reported cases of cronobacter infections is very low, but nevertheless there has been a slight increase recently. while the reported cases worldwide are few, it ne...

conclusions results of the current study confirmed that molecular methods such as pcr and lamp were much more sensitive, rapid, and specific than culturing to identify non-culturable coccoid forms of h. pylori in water. results ten copies of targeted dna were required for pcr detection whereas only five copies gave a positive reaction by lamp assay, with 100% specificity. of the 10 isolates ino...

results among the 225 isolates, 33.7% (76/225) and 18.1% (41/225) were resistant to imipenem and meropenem, respectively. of the 76 imipenem-resistant p. aeruginosa strains, 45 (59.2%) were positive for mbls, 34 (75%) strains carried the blaimp-1 gene, and 1 (2.2%) strain carried the blavim-2 gene. conclusions our results showed that there was a high frequency of imp-1 positive p. aeruginosa in...

conclusions a high rate of 25% and 24.13% of hbv dna was detected among patients with hodgkin and non-hodgkin lymphoma, respectively. results three out of 12 (25%) hodgkin samples and seven out of 29 (24.13%) non-hodgkin showed positive hbv dna results. the results of sequencing revealed that the d genotype was predominant among the positive hbv patients. interestingly an unpredictable amino ac...

conclusions human papillomavirus 16 as a high risk hpv was found to have the highest frequency among patients with anogenital warts. results out of 54 samples, 46 (85.18%) cases showed positive results for hpv dna. a total of 26 (56.6%) samples were males and 20 (43.4%) females while eight (14.81%) showed hpv negative results. overall, 37 (80%) patients had multiple sexual partners, and nine (2...

background: the emerge of rapid and accurate detection of meticillin-resistant staphylococcus aureus (mrsa) has been highlighted. objectives: the current study evaluated the prevalence of mec-a gene in biological specimens of various medical wards, in order to determine any possible relationship. patients and methods: using traditional culture methods, 250 isolates were detected. the prevalence...

conclusions: even, very low amount of csf-igg with good specificity (90%); could differentiate cases and controls (p = 0.000). the csf-igg test (sensitivity 70%, npv 28.8%) was weak for ruling out the m. pneumonia in cases. positive pcr was rare (2%) in csf of cases and controls but is more reliable for diagnosing the recent m. pneumonia infection. we prefer to assay the csf for both serology a...