نتایج جستجو برای: polymerarase chain reaction

تعداد نتایج: 627571  

Journal: :archives of clinical infectious diseases 0
soheila nasiri dermatology research center, shahid beheshti medical university, tehran, ir iran; dermatology research center, shohadaye tajrish hospital, tehran, ir iran fariba ghalamkarpoor dermatology research center, shahid beheshti medical university, tehran, ir iran asieh saberi dermatology research center, shahid beheshti medical university, tehran, ir iran parvaneh vesal dermatology research center, shahid beheshti medical university, tehran, ir iran

results in 12 patients (37%), human papilloma virus was detected by pcr method. in addition, there was no difference in distribution of patients with respect to affliction with human papilloma virus between high-risk and low-risk groups. there was no relationship between clinical manifestation and type of papilloma virus. materials and methods this cross-sectional study was conducted on 32 pati...

Journal: :avicenna journal of medical biotechnology 0

background: discovery of short cell free fetal dna (cffdna) fragments in maternal plasma has created major changes in the field of prenatal diagnosis. the use of cffdna to set up noninvasive prenatal test is limited due to the low concentration of fetal dna in maternal plasma therefore, employing a high efficiency extraction method leads to more accurate results. the aim of this study was to ev...

Journal: :iranian journal of public health 0
r bakhtiari mm soltan dallal jf mehrabadi s heidarzadeh mr pourmand

background: group  b  streptococcus   (gbs)  is  one  of  the  most  important  cause  of  morbidity  and  mortality  among  newborns  especially  in  developing  countries.  it  has  been  shown  that  the  screening  approach  rather  than  the  identification  of  maternal  clinical  risk  factors  for  early-onset  neonatal  gbs  disease  is  more  effective  in  preventing  early-onset  gb...

Journal: :iranian journal of pathology 0
reza ranjbar molecular biology research center, baqiyatallah university of medical sciences, tehran, iran afsar tabatabaee dept. of microbiology, zanjan branch, islamic azad university, zanjan, iran payam behzadi dept. of microbiology, college of basic sciences, shahr-e-qods branch, islamic azad university, tehran, iran rohollah kheiri water quality control office, alborz province water and wastewater company, karaj, iran

background: escherichia coli is a commensal-pathogenic organism, which includes a wide range of strains. despite several advanced molecular-genomic technologies for detecting and identifying different strains of e. coli, enterobacterial repetitive intergenic consensus polymerase chain reaction (eric-pcr) technique is a quick, sharp and cost effective fingerprint method. the major purpose of the...

Journal: :archives of pediatric infectious diseases 0
jalal mardaneh department of microbiology, school of medicine, gonabad university of medical sciences, gonabad, iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی گناباد (gonabad university of medical sciences) mohammad mehdi soltan dallal food microbiology research center, tehran university of medical sciences, tehran, iran; department of pathobiology, division of microbiology, school of public health, tehran university of medical sciences, tehran, iran; food microbiology research center, tehran university of medical sciences, tehran, iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (tehran university of medical sciences)

conclusions results showed that pif that is being consumed in iran is contaminated with this pathogen and can cause disease in infants; especially those hospitalized in neonatal intensive care units (nicus) and fed pif. the number of reported cases of cronobacter infections is very low, but nevertheless there has been a slight increase recently. while the reported cases worldwide are few, it ne...

Journal: :jundishapur journal of microbiology 0
parastoo chamanrokh department of biology, science and research branch, islamic azad university, tehran, ir iran; department of biology, science and research branch, islamic azad university, tehran, ir iran. tel: +98-9372727679 mohammad hassan shahhosseiny department of microbiology, shahre qods branch, islamic azad university, shahre qods, ir iran; iranian gene fanavar institute (igf), tehran, ir iran mahnaz mazaheri assadi department of biotechnology, iranian research organization for science and technology, tehran, ir iran taher nejadsattari department of biology, science and research branch, islamic azad university, tehran, ir iran davood esmaili department of microbiology, applied microbiology research center, baqiyatallah university of medical sciences, tehran, ir iran

conclusions results of the current study confirmed that molecular methods such as pcr and lamp were much more sensitive, rapid, and specific than culturing to identify non-culturable coccoid forms of h. pylori in water. results ten copies of targeted dna were required for pcr detection whereas only five copies gave a positive reaction by lamp assay, with 100% specificity. of the 10 isolates ino...

Journal: :jundishapur journal of microbiology 0
ramin abiri department of microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran; department of microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran. tel: +98-9122773648, fax: +98-8314276471 pantea mohammadi medical biology research center, kermanshah university of medical sciences, kermanshah, ir iran navid shavani department of microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran mansour rezaei department of biostatics and epidemiology, school of hygiene, kermanshah university of medical sciences, kermanshah, ir iran

results among the 225 isolates, 33.7% (76/225) and 18.1% (41/225) were resistant to imipenem and meropenem, respectively. of the 76 imipenem-resistant p. aeruginosa strains, 45 (59.2%) were positive for mbls, 34 (75%) strains carried the blaimp-1 gene, and 1 (2.2%) strain carried the blavim-2 gene. conclusions our results showed that there was a high frequency of imp-1 positive p. aeruginosa in...

Journal: :jundishapur journal of microbiology 0
kamyar makvandi infectious and tropical disease research center, health research institute, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran nastaran ranjbari department of pathology, imam khomeini hospital, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; department of pathology, imam khomeini hospital, ahvaz jundishapur university of medical sciences, ahvaz, ir iran. tel: +98-6133354389, fax: +98-6113361544 manoochehr makvandi department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran ali ashraf teimori department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran niloofar neisi department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran mojtaba rasti infectious and tropical disease research center, health research institute, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran

conclusions a high rate of 25% and 24.13% of hbv dna was detected among patients with hodgkin and non-hodgkin lymphoma, respectively. results three out of 12 (25%) hodgkin samples and seven out of 29 (24.13%) non-hodgkin showed positive hbv dna results. the results of sequencing revealed that the d genotype was predominant among the positive hbv patients. interestingly an unpredictable amino ac...

Journal: :jundishapur journal of microbiology 0
reza yaghoobi department of dermatology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran manoochehr makvandi virology department, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran nasim afshar department of dermatology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran nader pazyar department of dermatology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; department of dermatology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran. tel: +98-6188617133, fax: +98-6132921837 mojtaba hamidifard virology department, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran chia sharifpour virology department, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran

conclusions human papillomavirus 16 as a high risk hpv was found to have the highest frequency among patients with anogenital warts. results out of 54 samples, 46 (85.18%) cases showed positive results for hpv dna. a total of 26 (56.6%) samples were males and 20 (43.4%) females while eight (14.81%) showed hpv negative results. overall, 37 (80%) patients had multiple sexual partners, and nine (2...

Journal: :jundishapur journal of microbiology 0
afsaneh rajabiani department of pathology, shariati hospital, tehran university of medical sciences, tehran, ir iran fatemeh kamrani department of pathology, shariati hospital, tehran university of medical sciences, tehran, ir iran mohammad ali boroumand department of pathology, tehran heart center, tehran university of medical sciences, tehran, ir iran hiva saffar department of pathology, shariati hospital, tehran university of medical sciences, tehran, ir iran; department of pathology, shariati hospital, tehran university of medical sciences, ir iran. tel: +98-2184902195, fax: +98-2188633078

background: the emerge of rapid and accurate detection of meticillin-resistant staphylococcus aureus (mrsa) has been highlighted. objectives: the current study evaluated the prevalence of mec-a gene in biological specimens of various medical wards, in order to determine any possible relationship. patients and methods: using traditional culture methods, 250 isolates were detected. the prevalence...

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