Polygalacturonase inhibiting proteins (PGIPs) are major defensive proteins produced by plant cell walls that play a crucial role in pathogen resistance by reducing polygalacturonase (PG) activity. In the present study, a novel PGIP gene was isolated from tobacco (Nicotiana tabacum), hereafter referred as NtPGIP. A full-length NtPGIP cDNA of 1,412 bp with a 186 bp 5'-untranslated region (UTR), a...

Extracellular polygalacturonase (PG) production was estimated in vitro, using liquid cultures of three species of brown-rot decay fungi (Postia placenta, Gloeophyllum trabeum and Serpula incrassata), by cup-plate assay, assay of reducing sugars, and decrease in viscosity. Although all three experimental assays demonstrated that PG was induced by pectin in all three fungi, decrease in viscosity ...

Pectin lyase and polygalacturonase production by newly isolated Penicillium viridicatum strain Rfc3 was carried out by means of solid state fermentation using orange bagasse, corn tegument, wheat bran and mango and banana peels as carbon sources. The maximal activity value of polygalacturonase (Pg) (30U.g) was obtained using wheat bran as carbon source while maximal pectin lyase (Pl) (2000 U.g)...

Cellulase is an adaptive enzyme in most fungi (Reese and Levinson, 1952), although it is constitutive in cellulolytic bacteria (Hammerstrom et al., 1955). Many polysaccharases are adaptive in fungi, including: pentosanase (Simpson, 1954), polygalacturonase (Phaff, 1947), chitinase (Reynolds, 1954), dextranase (Hultin and Nordstr6m, 1949), and xylanase and mannanase (S0rensen, 1952). Since many ...

Polygalacturonase-inhibiting protein (PGIP), belonging to a group of plant defence proteins, specifically inhibits endopolygalacturonases secreted by pathogens. Herein, we showed that purified GhPGIP1 is a functional inhibitor of Verticillium dahliae and Fusarium oxysporum f. sp. vasinfectum, the two fungal pathogens causing cotton wilt. Transcription of GhPGIP1 was increased in cotton upon inf...

The objective of the study was to determine changes in activities enzymes degrading cell wall response hot water, calcium chloride (CaCl2) and 1-methlylcyclopropene (1-MCP) treatments during post-harvest storage sweet cherries. For this purpose, 0.5, 1, 5 μl L-1 1-MCP, 1% 2% CaCl2 45, 50, 55 oC waters were applied As a result conducted with wall, it observed that very significant increases occu...

The aim of this study was to investigate the effect enzyme hydrolysis jackfruit pulp using two commercial pectinase solutions with different polygalacturonase activities. A 2³ factorial design, three repetitions at central point, used establish optimal conditions hydrolysis, evaluating time (20 - 100 min), temperature (30 60ºC) and concentration (0.01 0.09%). dependent variables viscosity, yiel...

Botrytis allii and Sclerotium cepivorum, which respectively cause neck rot and white rot of onions, produce a range of cell-wall-degrading enzymes in onion tissue (Hancock et al., 1964; Beck, 1976). The two fungi have been grown on liquid media using purified onion cell walls, prepared by an adaptation of the method of Jarvis et al. (1977), as the sole carbon source for B. allii and the major c...