The healing potential of scaffolds for tissue engineering can be enhanced by combining them with genes to produce gene-activated matrices (GAMs) for tissue regeneration. We examined the potential of using polyethyleneimine (PEI) as a vector for transfection of mesenchymal stem cells (MSCs) in monolayer culture and in 3D collagen-based GAMs. PEI-pDNA polyplexes were fabricated at a range of N/P ...

Intracellular protein delivery may provide a safe and non-genome integrated strategy for targeting abnormal or specific cells for applications in cell reprogramming therapy. Thus, highly efficient intracellular functional protein delivery would be beneficial for protein drug discovery. In this study, we generated a cationic polyethyleneimine (PEI)-modified gelatin nanoparticle and evaluated its...

Rapid methyl parathion detection was measured using a fabricated glassy carbon electrode (GCE) sensor designed the layer-by-layer (LBL) method. Multilayer assemblies were developed on by alternating depositions of anions and cations in which Dawson Type Polyoxometalate ?-K 6 [(P 2 W 18 O 62 ·H O)]·14H (?P POM) polyethyleneimine (PEI) stabilized silver nanoparticles (?PEI-AgNPs) acted as cations...

Gene therapy combined with chemotherapy to achieve synergistic therapeutic effects has been a hot topic in recent years. In this project, the human tumor necrosis factor-related apoptosis-inducing ligand-encoding plasmid gene (TRAIL) and doxorubicin (Dox)-coloaded multi-functional nanocarrier was constructed based on the theory of circulation, accumulation, internalization, and release. Briefly...

Magnetofection is a nanoparticle-mediated approach for transfection of cells, tissues, and tumors. Specific interest is in using superparamagnetic iron oxide nanoparticles (SPIONs) as delivery system of therapeutic genes. Magnetofection has already been described in some proof-of-principle studies; however, fine tuning of the synthesis of SPIONs is necessary for its broader application. Physico...

Dye wastewater containing bisphenol A (BPA) and dyes as pollutants has not been adequately studied. Our previous study revealed that thermoplastic polyurethane (TPU) nanofiber membranes (NFMs) modified by the addition of polyethyleneimine (PEI) polydopamine (PDA) satisfactorily adsorb dyes. Herein, we first optimized synthesis conditions for such membranes, noting a PEI/PDA monomer ratio 2:2 de...

The efficient delivery of therapeutic genes into cells of interest is a critical challenge to broad application of non-viral vector systems. In this research, a novel TPGS-b-(PCL-ran-PGA) nanoparticle modified with polyethyleneimine was applied to be a vector of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and endostatin for cervical cancer gene therapy. Firstly, a novel biod...

Objectives: Gold nanoparticles are very popular metallic nanomaterials and they have a wide spectrum of biomedical applications. This study was aimed to the production stable monodisperse polyethyleneimine (PEI) polyethylene glycol (PEG) coated gold (AuNP20 AuNP50), investigation their in vivo biochemical effects BALB/c mice. Methods: were synthesized surfaces modified by PEI PEG. All necessary...

Positively charged polyelectrolytes hold significant potential as materials for creating antibacterial coatings. We examined the physicochemical and mechanical properties of macromolecules in water solutions coatings series branched polyethyleneimine (PEI) linear polydiallyldimethylammonium chloride (PDADMAC) with different molecular weights. The microbiological study was conducted to analyze b...

An efficient transfection is a crucial step for the introduction of epigenetic modification in host cells, and there need an optimized process individual model systems separately. Mouse pancreatic αTC1-6 which act as attractive system cell reprogramming diabetes treatment, were transiently transfected with two different methods: chemical method polyethyleneimine (PEI) nucleofection physical met...