We have constructed a drug delivery vehicle that targets the epidermal growth factor receptor (EGFR) and its mutant isoform EGFRvIII. The monoclonal antibody, cetuximab, previously known as C225, which binds to both EGFR and EGFRvIII, was covalently linked via its Fc region to a fifth-generation (G5) polyamidoamine dendrimer containing the cytotoxic drug methotrexate. As measured by mass spectr...

Functional, three-dimensional (3-D) nanostructures and microstructures on surfaces can serve as excellent molecular templates for applications in optoelectronics and biotechnology. Coupling stable, amphiphilic films with hydrophobic biological molecules can yield biomimetic interfaces able to reproduce biological functions in vitro. Recently, thin films of amphiphilic molecules have been used t...

Unlike antibiotics and heavy metals, nucleic acids exist in the aquatic environment as a part of prokaryotic eukaryotic microorganisms (bacteria, fungi, etc.) rather than free form. In this regard, most important primary stage sample preparation an object for quantitative analysis DNA RNA natural wastewaters includes membrane ultrafiltration aqueous sample, followed by its sorption preconcentra...

Highly branched, functionalized polymers have potential to act as efficient drug carrier systems. Dendrimers are ideal candidates among model hyperbranched polymers because of their well-defined structure and high density of functional groups. Using ibuprofen as a model drug, we studied the interaction between the drug and Polyamidoamine (PAMAM) dendrimers (generations 3 and 4 with --NH2 functi...

Introduction Paramagnetic contrast agents targeted to cell membrane receptors are currently of great interest for molecular imaging with MRI [1]. In addition to the advantages of imaging endogenous receptors, expressed for example on tumor cells, receptor targeted MRI has also been proposed as a method for imaging transgene expression, using defined cell surface proteins such as transferrin rec...