We have further characterized the sensitivity and specificity of SYPRO Orange protein gel stain and SYPRO Red protein gel stain with native and 2-dimensional polyacrylamide gels and for staining gels prior to Western blot analysis. We found that nucleic acids are not stained by the SYPRO protein gel stains, in contrast to results obtained with commonly used silver staining techniques. Bacterial...

We attempted to improve a protocol to allow for the consistent extraction of total RNA from prokaryotic cells. Total RNA was isolated from Escherichia coli B23 and yeast cells using Trizol. In the comparison of two different protocols it was found that performing the phase separation independent from the bead bashing results in increased yield and purity of the RNA. We also attempted to improve...

We used polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis to screen the most frequent variants (A, B, C, and E) found in the bovine kappa-casein gene. The PCR products (453 bp) were heat-denatured, loaded onto nondenaturing polyacrylamide gels, and silver-stained. Each variant yielded patterns clearly distinguishable from the others. Optimal conditions for th...

Low oil extraction and early high water production are caused in part by reservoir heterogeneity. Huge quantities of prevalent issues that happen older reservoirs. Polyacrylamide polymer gel systems have been frequently employed as plugging agents heterogeneous reservoirs to regulate output increase sweep efficiency. classified into three classes depending on their composition application condi...

Phosphoenolpyruvate carboxykinase was purified from mitochondria of guinea-pig liver by affinity chromatography on GMP-Sepharose. The enzyme was purified 100fold to a high degree of electrophoretic homogeneity asjudged by detection of a single protein band on sodium dodecyl sulphate/polyacrylamide gels. The yield was about 16%. The Mr of the purified enzyme was estimated to be 68 500 + 680 by a...

We describe the use of polyacrylamide gel electrophoresis to estimate chain lengths of double- and single-stranded DNA molecules in the size range 20-1000 base pairs (or nucleotides). Double-stranded DNA molecules of known length produced either by organic synthesis or by restriction endonuclease digestion of viral DNAs were used as standards. The relative electrophoretic mobilities of these st...

Employing a 3 step purifICation procedure follicle stimulat ing hormone (FSH}-suppressing factor, desJgn.lCd "inbibin, w" purified to homogeneity from human IItmin.1 plasma(HSP). The ethanol/etherutract ofHSP was chrorMtoanpbcd 00 Sep~du G-IOO column. The' biologically adive fraction duted was electrophoresed on Pevilum block lODe electropbo~i. and sut-qucntly isolIIted in pure fonn on a OEAE.(...

1. Human pancreatic juice contains amylase, lipase, ribonuclease, deoxyribonuclease, proelastase, procarboxypeptidase A, procarboxypeptidase B, chymotrypsinogen, and trypsinogen, as well as a tqqsin inhibitor. The level of the inhibitor is such that 1 ml of pancreatic juice containing 4 mg of protein will inhibit about 0.08 mg of bovine trypsin. The inhibitor is a small, basic protein, soluble ...

One of the major proteins secreted by Sertoli cells (Band 3) has a molecular weight on sodium dodecyl sulfate-polyacrylamide gels which corresponds to rat serum transferrin. The preparation of Sertoli cell-secreted proteins contains an iron-binding protein with electrophoretic properties on native polyacrylamide gels similar to serum transferrin. Antibodies against Sertoli cell-secreted protein...

An isoelectric focusing method capable of resolving all groups of storage protein of the wheat seed, including the most basic low molecular weight glutenin (LMWG), was developed. Ultrathin polyacrylamide gels were used after drying and rehydration with 8 M urea, 50 mM DTE and 2.4% carrier ampholytes (pH 4-9). Densitometric scanning of the isoelectric focusing gels permitted a more accurate and ...