We describe a general methodology for fluorescent labelling of peptide conjugates of phosphorodiamidate morpholino oligonucleotides (PMOs) by alkyne functionalization of peptides, subsequent conjugation to PMOs and labelling with a fluorescent compound (Cy5-azide). Two peptide-PMO (PPMO) examples are shown. No detrimental effect of such labelled PMOs was seen in a biological assay.

The interrupter technique is a non-invasive method for measuring airway calibre. Since the calculation of interrupter resistance (Rint) is critically dependent upon the analysis of the mouth pressure/time (Pmo(t)) curve obtained after flow interruption, we wanted to assess the relative merits of four different analyses of Pmo(t) curves, obtained under basal conditions and following methacholine...

• The PMO has three distinctive sets of knowledge brokering roles. Bottom-up roles promote experiential learning to inform decision making. Top-down maintain deliberate assist continuous improvement. Horizontal facilitate synchronisation help intra level learning. collectively contribute better governance. Organisations often suffer from flow gaps between operational and strategic management le...

The synthesis of two biocatalysts based on a commercial Candida antarctica lipase B, CALB enzyme (E), physically immobilized silica supports, was carried out. first support periodic mesoporous organosilica (PMO) and the second one modified with octyl groups (octyl-MS3030). maximum load 122 mg enzyme/g PMO 288 octyl-MS3030. In addition, biocatalytic efficiency corroborated by reaction tests hydr...

Several studies have reported tissue blood flow and tissue oxygen tension during anesthesia, whereas there are few reports that discuss the relationship between tissue blood flow and tissue oxygen tension. The goal of this study was to investigate the relationship between these two variables before and after stellate ganglion block (SGB). We utilized 8 male Japan White rabbits. Anesthesia was m...

primarily driven by hospitalizations related to fractures resulting from osteoporosis; costs range from $15,500 for any osteoporosis-related hospitalization to $17,383 for an osteoporosis fracture-related admission. In the absence of a fragility fracture, a diagnosis of osteoporosis can be made with a measurement of BMD. The expression of BMD, compared with the reference mean, is known as the T...

The aerobic oxidation of alcohols was efficiently completed in high conversion and selectivity using Cu3/2[PMo12O40]/SiO2 as catalyst under mild reaction condition. This reaction provides a new environmentally friendly rout to the conversion of alcoholic function to carbonyl groups.