نتایج جستجو برای: plasmid by the restriction enzymes then
تعداد نتایج: 17032277 فیلتر نتایج به سال:
A critical and difficult part of characterizing restriction enzymes and methylases is the identification of recognition sequences. To simplify this process, we have developed a plasmid transformation method along with a computer program named RM search that determines the exact recognition sequences for given restriction and modifica-
Interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. Recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (HCL). The aim of the present study is to apply yeast strain for secreting human IFNα2b fol...
the present study was carried on to investigate the use of face-keeping strategies in reaction to complaints by male and female in two different cultures, persian and american english. to see if apology strategies are affected by cultural, contextual preferences and gender of interlocutors in two distinct languages of persian and american english. the hypotheses to be tested were 1) ifids, acce...
background and objectives: varicella–zoster virus (vzv) is a human herpes virus that usually attacks young children and commonly causes chicken pox (varicella). following primary infection, a lifelong latent infection is established. the virus often reactivates during adulthood or senesces to cause shingles (zoster). little is known regarding the genotypes of varicella in kuwait. the aim of thi...
In molecular biology for cloning experiments, plasmid, a circular molecule is used as vector (carrier) to insert a desired gene or DNA fragment by ligating into it after digestion of both with the compatible restriction enzymes. Then by transforming with a host (generally E. coli) strain of desired traits the screening for appropriate clone is done. A plasmid is having selectable marker in the ...
The plasmid profile and BamHI restriction pattern of 17 sorbitol-negative and 1 sorbitol-positive French Yersinia ruckeri strain of the American type strain were studied. The 17 sorbitol-negative strains and the American strain harbored a 62-megadalton (MDa) plasmid with an identical BamHI restriction pattern. Southern hybridization indicated that this 62-MDa plasmid is common among these vario...
as some definitions show, idioms are expressions whose meanings cannot be obtained from individual words. in every society, people use their own conceptions and feelings through different idioms and expressions. so every culture and society has their own idioms. some scholars proposed methods for translating idioms but baker’s strategies are very important and constructive. this research tried ...
investigation the archetype of mother can help the reader to understand poes works, especially his fiction, better, if not fully. motivated by internal and external drives to get into the universe in its manifold form poe was impelled to art and, from various modes of art, to symbolism. how much was poe successful to produce works of art has been a matter of dispute among critics. however, ther...
Type II restriction-modification (R-M) systems encode a restriction endonuclease that cleaves DNA at specific sites, and a methyltransferase that modifies same sites protecting them from restriction endonuclease cleavage. Type II R-M systems benefit bacteria by protecting them from bacteriophages. Many type II R-M systems are plasmid-based and thus capable of horizontal transfer. Upon the entry...
Abstract: Coxsackievirus B3 (CVB3) is the most common agent known to cause viral myocarditis. The viral genome encodes a single polyprotein that is cleaved to produce several proteins by virally encoded proteases. Most of this proteolytic processing is catalyzed by a cysteine protease called 3C. The 3C protease plays major role in viral replication and cellular damage. To understand the mecha...
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