mycoplasma and ureaplasma species are the most serious contaminants of cell cultures and this remains one of the major problem encountered in biological research using cell cultures. the aim of this study was to investigate the rate of mycoplasma and a total of 100 different cell culture specimens including, r228, hela, vero, mdck, l20, rd and hep2 were collected. then the specimens were invest...

introduction: some studies have demonstrated the potential of herbal drugs for the treatment of various diseases associated with impaired vascular nitric oxide (no). for diagnosis of the mechanism of these herbal plants, it seems necessary to evaluate the herbal extracts on the endothelium cell. the aim of the study was to investigate the effect of extract of trigonella foenum-graecum (t.foenum...

Plant secondary metabolites are well-recognized medicinally important compounds. Gossypol is an plant metabolite with several medicinal properties. Carbon nanotubes (CNTs) allotropes of carbon diverse applicability in chemical, physical, and biological sciences due to their high surface area. The current study demonstrates the enhancement gossypol production cotton cell suspension culture media...

Plant biotechnology has emerged as a powerful tool of crop improvement and has aroused a great deal of interest in many countries because of its application to agriculture and horticulture. We report here the application of a number of in vitro plant breeding techniques such as micropropagation, in vitro flowering, in vitro pollination and fertilisation, anther culture, somatic embryogenesis, a...

the natural lignan podophyllotoxin, a dimerized product of two phenylpropanoid moieties which occurs in a few plant species, is a pharmacologically important compound for its anticancer activities. optimization of the in vitro cultures might lead to a renewable source of lignans for medicinal uses. in order to maximize efficiency of podophyllotoxin accumulation via in vitro cultures, the effect...

members of the genus bacillus are among the most promising candidates for biological control of plant pathogenic bacteria. in this study, cell free supernatants of three strains b. subtilis utb96, b. subtilis utb1 and b. subtilis utb70 against aspergillus flavus r5 were investigated using dual culture assay. strain b. subtilis utb96 showed the highest inhibition effect. thin lyer chromatography...

The present study describes the major content and antimicrobial activity of Azadirachta indica cell culture extracts. Major constituents of cell culture extracts were evaluated by HPLC and chromatographic analysis revealed that highest concentrations of azadirachtin (0.0069 % dry wt) synthesised by multiple shoot cultures. The results showed that multiple shoot cultures synthesised ~17-fold mor...

In order to investigate new metabolites which are only induced in a plant callus infected by a pathogenic fungus, dual cultures with combinations of 10 species of fungi and 6 plant cell lines from different species were established. Among the combinations tested, the methanolic extract of a dual culture consisting of a plant cell line, Phytolacca americana and a fungus, Botrytis fabae showed a ...

Agrobacterium overgrowth is a common problem in Agrobacterium-mediated plant transformation. To suppress the Agrobacterium overgrowth, various antibiotics have been used during plant tissue culture steps. The antibiotics are expensive and may adversely affect plant cell differentiation and reduce plant transformation efficiency. The SacB-SacR proteins are toxic to most Agrobacterium tumefaciens...

Plant tissue culture, or the aseptic culture of cells, tissues, organs, and their components under defined physical and chemical conditions in vitro, is an important tool in both basic and applied studies as well as in commercial application. It owes its origin to the ideas of the German scientist, Haberlandt, at the beginning of the twentieth century. The early studies led to root cultures, em...