Cutinases are hydrolytic enzymes which catalyzes esterification and trans-esterification reactions that make them highly potential industrial biocatalyst. In the present investigation microorganisms showing cutinase activity were isolated from plant samples. The strain showing maximum cutinase activity was identified by 18S rDNA sequencing as Aspergillus sp. RL2Ct and was selected for further s...

A bacterial strain of marine origin showing production of poly(g-glutamic acid) (PGA) has been identified by taxonomical and 16S rRNA studies as Bacillus subtilis. A sequential optimization approach was applied for improving the PGA production. The effect of carbon sources, nitrogen sources and pH on the production of PGA was investigated by one factor-at-a-time method. Plackett-Burman design w...

Lipase production by Candida rugosa was carried out in submerged fermentation. Plackett-Burman statistical experimental design was applied to evaluate the fermentation medium components. The effect of twelve medium components was studied in sixteen experimental trials. Glucose, olive oil, peptone and FeCl3.6H2O were found to have more significance on lipase production by Candida rugosa. Maximum...

As system complexity grows, cycle-accurate simulation experiments become inordinately time consuming. Most approaches to accelerating architectural simulation model only portions of an application in detail. Detailed simulation begins with architectural state from earlier portions of the execution. Constructing this requires fast-forwarding: modeling effects of earlier instructions without mode...

Piriformospora indica, a member of basidiomycota is an axenically cultivable endophytic fungus which exerts plant growth promoting effects on its host plant. P. indica is known to produce α-amylase and protease. Since the organism exhibits beneficial role in plant growth promotion, achieving high biomass is immensely essential. Hence to enable the commercial production, screening of medium comp...

This paper describes and discusses a Plackett-Burman experiment aimed at increasing supermarket sales of a top-selling national magazine. The experiment involved 10 factors relating to in-store advertising and the location of the magazine within the store. We discuss issues including choice of factors, alternative designs, sample-size determination (number of test stores and the length of the t...

R.Bęczkowski, Institute of Mechanical Technology, Czestochowa University of Technology, Poland Hardness is one of the components responsible for the resistance to wear. The development of new materials with hardness surface more than 65 HRC is possible with use welding technologies. High chromium cored wires belong to welding materials that are often used to cladding to protect surface. This ar...

Many Trichoderma species are able to produce 6-pentyl-α-pyrone (6-PP), a lactone with coconut-like aroma. In the present work, several culture parameters were studied to enhance the production of 6-PP by Trichoderma harzianum 4040 in solid-state fermentation. Green coir powder added to a nutrient solution was used as support material for fermentation. A Plackett-Burman screening technique was a...

The main effects of six experimental factors on the efficiency of HS (headspace) extraction in headspace gas chromatography--flame ionization detector (HS-GC-FID) determination of twenty organic solvents routinely used in production of pharmaceuticals were obtained on the basis of the results of experiments carried out according to the Plackett-Burman factorial design. The effects were used as ...