Abstract Background and Aims Autosomal-dominant polycystic kidney disease (ADPKD) is the most common inherited renal disorder characterized by progressive bilateral cysts development extrarenal phenotype, i.e. liver and/or pancreatic cysts, intracranial aneurism, hernias, mitral valve prolapse diverticulosis. More than 90% of patients harbour heterozygous pathogenic variant in PKD1 or PKD2 gene...

Abstract Background and Aims Renal cystic disease (RCD) includes a spectrum of disorders with heterogenous clinical presentation. Among RCD are autosomal dominant polycystic kidney (ADPKD), recessive (ARPKD), ciliopathies, HNF1B-nephropathy, congenital anomalies the kidneys urinary tract (CAKUT). Diagnosis is based on criteria, yet, given extreme genetic heterogeneity phenotypic overlap among d...

There are many techniques to knock out directed genes in bacteria, some of which have been described in Salmonella species. In this study, a combination of SOEing PCR method and the λ Red disruption system were used to disrupt phoP gene in wild type and standard strains of Salmonella typhimurium. Three standards PCR and one fusion PCR reactions were performed to construct a linear DNA including...