Colour changes in Gradia Direct™ composite after immersion in tea, coffee, red wine, Coca-Cola, Colgate mouthwash, and distilled water were evaluated using principal component analysis (PCA) and the CIELAB colour coordinates. The reflection spectra of the composites were used as input data for the PCA. The output data (scores and loadings) provided information about the magnitude and origin of ...

Abbreviations: A0: Cross-Section; BDNF: Brain Derived Growth Factor; BMP: Bone Morphogenetic Proteins: CGF: Concentrated Growth Factors: EGM: Endothelial Growth Medium:EBM-2: Endothelial Basal Medium 2; F: Load; FGM: Fibroblast Growth Medium; FBM: Fibroblast Basal Medium; H: Height; H0: Initial Height; HE: Hematoxylin And Eosin Staining; HOB: Human Osteoblasts; HRP: Horseradish Peroxidase; HUVE...

Over the last few decades, many scientists considered microalgae as promising actors for future biofuels because of the high lipid productivity inside their cells. Moreover, much attention has been paid to algal lipids as they can be used in biodiesel production. In this study, we optimized the different suitable conditions such as incubation time, incubation temperature, Dimethylesulfoxide and...

THE great disadvantages of the standard staining methods for mitochondria are that they are very complicated and cannot be applied to different tissues until suitable times for the action of the various stains have been worked out by a long process of trial and error. The following method is easy to control and apply. Destaining and restaining are quickly carried out, times may be worked out on...

A clonal strain of epithelial cells (designated MH(1)C(1)) has been established from the transplantable Morris hepatoma No. 7795. The cells have maintained distinctive morphology throughout more than 20 subcultures (split 1:5) at 2- to 4-week intervals in supplemented Ham's F 10 medium. They contain many highly refractile, round, cytoplasmic bodies which stain bright red with Oil Red O. The pop...

Foci of cells infected with human cytomegalovirus were noted to stain more intensely than uninfected cells with neutral red, and this provided the basis for development of a plaque assay and plaque reduction neutralization test for cytomegalovirus. Plaques demonstrable by neutral red staining could be counted at 8 days after infection; thus, results could be obtained earlier than for plaque ass...

Interstitial fibrosis is commonly measured by histology. The Masson trichrome stain is widely used, with semiquantitative scores subjectively assigned by trained operators. We have developed an objective technique combining Sirius Red staining, polarization contrast microscopy, and automated analysis. Repeated analysis of the same sections by the same operator (r = 0.99) or by different operato...