This study focused on the optimization of xylitol production from sugarcane bagasse by using response surface methodology. Xylitol was produced through a series processes, firstly, ultrasound-assisted mild alkaline pretreatment for xylan extraction followed enzymatic hydrolysis to xylose enzyme β-1,4-xylanase and finally microbial fermentation yeast (Candida guilliermondii), bacteria (Corynebac...

mortality in compromised hosts. Fukazawa et al. designed a slide agglutination scheme which uses 10 factor sera, 1, 4, 5, 6, 8, 9, 11, 13, 13b, and 34, whereby it is possible to make a rapid and accurate identification of seven medically important Candida species as a commercially available serum factor kit including the 10 factor sera (Candida Check). A structural model of Candida mannans, ind...

BACKGROUND For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized enzymes. RESULTS Two Pichia pastoris cell surface display vectors based on the flocculation functional domain of FLO with its own secretion signal...

Fungal infections due to Candida species represent an important cause of nosocomial bloodstream infections. We report a large pseudo-outbreak of Candida guilliermondii fungemia that occurred in a university hospital in Brazil. C. guilliermondii was identified in 64 (43%) of the 149 blood samples drawn between June 2003 and July 2004. The samples were from patients in different wards of the hosp...

BACKGROUND Isolated pulmonary valve infective endocarditis caused by Candida is rare in chronic hemodialysis patients. The 2009 Infectious Diseases Society of America guidelines suggest the combined use of surgery and antibiotics to treat candidiasis; however, successful nonsurgical treatment of Candida endocarditis has been reported. CASE PRESENTATION A 63-year-old woman with end-stage kidne...

Eleven of 13 Enterobacteriaceae species tested grew in moist stored wheat, highlighting a potential risk of this energy-saving airtight storage method. When Hansenula anomala was coinoculated, all Enterobacteriaceae species were significantly inhibited after 2 months of storage, six of them to below the detection limit.

To express bovine chymosin in yeast, we amplified the prochymosin gene from the plasmid pMD18T-Prochy by PCR, and then cloned the gene into the expression vector pPICZaA, resulting in pPICZaA-Prochy. Pichia pastoris GS115 was used as host cells. Integration of the prochymosin cDNA into the Pichia pastoris genome was confirmed by PCR and sequencing analysis. Chymosin was expressed in Pichia past...

BACKGROUND Candida spp. are dimorphic fungi in the family Cryptococcaceae. Infections with Candida spp. are usually rare conditions in dogs, but immunocompromised patients have a higher risk for developing invasive candidal infections. CASE PRESENTATION A 5-year-old male Boxer, positive to Leishmania infantum, was referred to the Veterinary Teaching Hospital of the Department of Veterinary Me...

OBJECTIVES Heat shock protein 90 (hsp90) is targeted by the humoral response in invasive candidiasis. This paper tests for synergy between caspofungin and efungumab--a human antibody fragment against hsp90. METHODS The MIC-0, MIC-2 values and FICI were determined for a range of yeasts against efungumab and caspofungin. These yeasts were injected intravenously into mice with: 100 microL of sal...