Cutaneous field of cancerization (CFC) is caused in part by the carcinogenic effect of the cyclobutane pyrimidine dimers CPD and 6-4 photoproducts (6-4PPs). Photoreactivation is carried out by photolyases which specifically recognize and repair both photoproducts. The study evaluates the molecular effects of topical application of a film-forming medical device containing photolyase and UV filte...

The purpose of this research is to investigate gold nanoparticles as a potential scaffold for the characterization of DNA damage and repair using atomic force microscopy (AFM) techniques. The procedure consists of functionalizing the surface of gold nanoparticles with DNA which are then immobilized onto amine modified silicon or glass surfaces. Our objective is to examine various lesions in ind...

enon, photoreactivation, was later observed in several other organisms such as bacteriophages (DULBECCO 1950), bacteria and fungi of the genera Penicillium and Neurospora ( KELNER 1952). Reactivation of cells inactivated by ultraviolet irradiation was also obtained by means of pyruvic acid and other acids of the Krebs cycle (THOMPSON, MEFFERD and WYSS 1951; HEINMETS et al. 1954). Up to the end ...

Repair of cyclobutyl pyrimidine dimers (CPDs) in DNA is essential in most organisms to prevent biological damage by ultraviolet (UV) light. In higher plants tested thus far, UV-sensitive strains had higher initial damage levels or deficient repair of nondimer DNA lesions but normal CPD repair. This suggested that CPDs might not be important for biological lesions. The photosynthetic apparatus h...

Chlamydomonas reinhardtii is a prospective model system for understanding molecular mechanisms associated with DNA repair in plants and algae. To explore this possibility, we have developed an in vitro repair system from C. reinhardtii cell-free extracts that can efficiently repair UVC damage (Thymine-dimers) in the DNA. We observed that excision repair (ER) synthesis based nucleotide incorpora...