Citation: Cieplik F, Steinwachs V-S, Muehler D, Hiller K-A, Thurnheer T, Belibasakis GN, Buchalla W and Maisch T (2018) Phenalen-1-one-Mediated Antimicrobial Photodynamic Therapy: Antimicrobial Efficacy in a Periodontal Biofilm Model and Flow Cytometric Evaluation of Cytoplasmic Membrane Damage. Front. Microbiol. 9:688. doi: 10.3389/fmicb.2018.00688 Phenalen-1-one-Mediated Antimicrobial Photody...

1. Photodynamic therapy could be an alternative treatment for highly prevalent local antibiotic-resistant pathogens. 2. Photodynamic inactivation using toluidine blue O was observed on both reference strains and clinical samples, including methicillin-resistant Staphylococcus aureus, e x t e n d e d s p e c t r u m β l a c t a m a s e – p r o d u c i n g Escherichia coli and Klebsiella pneumoni...

Although reports of the photodynamic inactivation of viruses appeared in 1928, long before chemotherapeutic antiviral drugs, the first clinical trial in humans-the topical treatment of herpes genitalis-did not take place until the early 1970s. Trials were discontinued due to the transformation of healthy cells and concomitant incidence of Bowen's disease in some patients, probably due to the mi...

The synergism of low-frequency electromagnetic field treatment and photodynamic effect on killing of human cancer cells is presented. The weak pulsating electromagnetic field (PEMF) generated by Helmholtz coils in the mT range influences the permeability of cell membranes for photosensitizers. Several types of sensitizers were excited by visible light during incorporation without and with two k...

Cucumber (Cucumis sativus L. cv Poinsette) plants were sprayed with 20 millimolar 5-aminolevulinic acid (ALA) and then incubated in dark for 14 hours. Upon transfer to sunlight ( approximately 800 watts per square meter) the plants died after 5 hours of exposure due to photosensitization reaction of metalloporphyrins. Due to the photodynamic damage, photosystem II (PSII), photosystem I (PSI), a...

Photodynamic therapy involves administration of a photosensitizing drug and its subsequent activation by irradiation with a light source at wavelengths matching the absorption spectrum of the photosensitizer. In many countries around the world, topical photodynamic therapy has been approved for treatment of cutaneous oncologic conditions such as actinic keratosis, Bowen's disease, and superfici...

BACKGROUND Increasing resistance of oral pathogens to conventional antibacterial agents has resulted to find alternative therapies to overcome resistance development problems; hence this in vitro study was carried out to investigate the efficacy of photoelimination of Streptococcus mutans with two methods of photodynamic and photothermal therapy. METHODS Standard Suspensions of S. mutans were...

Hidradenitis suppurativa has been described as a chronic, recurrent, and disabling inflammatory disease involving the entire hair follicle. Several treatments, including photodynamic therapy, have been used, but the results have been inconsistent and recurrence is high. In this prospective study, we evaluated disease severity, quality of life, and treatment tolerance in 5 patients with moderate...

The photodynamic therapy of cancer is contingent upon the sustained generation of singlet oxygen in the tumor region. However, tumors of the most metastatic cancer types develop a region of severe hypoxia, which puts them beyond the reach of most therapeutic protocols. More troublesome, photodynamic action generates acute hypoxia as the process itself diminishes cellular oxygen reserves, which ...

Singlet oxygen generated in a type II photodynamic action, due to its limited lifetime (1 μs) and reactive distance (<10 nm), could regulate live cell function nanoscopically. The genetically-encoded protein photosensitizers (engineered fluorescent proteins such as KillerRed, TagRFP, and flavin-binding proteins such as miniSOG, Pp2FbFPL30M) could be expressed in a cell type- and/or subcellular ...