The function of the pre-B cell receptor (pre-BCR) during B cell differentiation is not precisely defined. To investigate the pre-BCR receptor activity, we have established pre-BCR-positive pre-B cell lines that are able to differentiate into immature B cells in vitro. Antibody cross-linking of the pre-BCR induced apoptosis and differentiation accompanied with tyrosine phosphorylation. A specifi...

The synthesis and analytical applications of the new heterocyclic ligand are described. The complexation reaction of the ligand with some cations was studied in aqueous methanol at room temperature using the spectrophotometric method. Results revealed that only the absorption spectra of the Fe (III)–ligand complex show a high redshift of the absorption maximum in the aqueous methanol soluti...

According to the GLOBOCAN, colorectal cancer (CRC) is second leading cause of deaths. Thus, new therapies for CRC are urgently needed. In search drug, our team studied a series cyanide gold (III) complexes (International Patent No: WO219008013A1). Our initial studies on cancerous RAS-3T3 and non-cancerous NIH-3T3 murine fibroblasts showed that complex known as TGS121, [Au(CN)4]2 (ClO2)Na, has s...

The asialoglycoprotein (ASGP) receptor on Hep G2 cells undergoes constitutive recycling and ligand endocytosis in the presence of phorbol dibutyrate, at a 50% reduced rate relative to control cells (Fallon, R. J., and Schwartz, A. L. (1986) J. Biol. Chem. 261, 15081-15089). The relevance of receptor phosphorylation to these events was investigated by selective immunoprecipitation of surface rec...

pp125FAK (focal adhesion kinase) a protein tyrosine kinase that may mediate cellular responses to adhesion, is activated and tyrosine-phosphorylated when platelets adhere to fibrinogen via the integrin, αIIbβ3. To determine whether either of the cytoplasmic tails of αIIbβ3 regulates FAK phosphorylation, CHO cells were stably transfected with αIIbβ3 or various cytoplasmic tail truncation mutants...

cAMP receptors mediate some signaling pathways via coupled heterotrimeric G proteins, while others are G-protein-independent. This latter class includes the activation of the transcription factors GBF and STATa. Within the cellular mounds formed by aggregation of Dictyostelium, micromolar levels of cAMP activate GBF function, thereby inducing the transcription of postaggregative genes and initi...