By T. J. GREENWALT AND V. E. AYERS T HE DISTRIBUTION of phosphate compounds in acid extracts of the erythrocytes of infants has heretofore been investigated only by fractional analysis for inorganic, total and hydrolyzable phosphates.1 In our previous work hydrolysis of trichloracetic acid extracts of cord blood erythrocytes was used to characterize the following fractions : ( 1 ) labile phosph...

Fructosamine 3-kinase (FN3K), an enzyme initially identified in erythrocytes, catalyses the phosphorylation of fructosamines on their third carbon, leading to their destabilization and their removal from protein. We show that human erythrocytes also contain FN3K-related protein (FN3K-RP), an enzyme that phosphorylates psicosamines and ribulosamines, but not fructosamines, on the third carbon of...

An inositol-requiring strain of Neurospora crassa was labelled during growth in liquid medium with [3H]inositol, and the levels of inositol phosphates and phosphoinositides were determined under inositol-sufficient and inositol-starved conditions. Because the mutant has an absolute requirement for inositol, the total mass of inositol-containing compounds could be determined. Inositol-containing...

Rare earth elements (REE) speciation in laterites are commonly estimated from sequential extraction procedure (SEP). In this study, the selectivity of a 5-steps SEP protocol was evaluated on individual synthetic Ce-material and applied to well-characterized lateritic profile developing over granite bedrock (Madagascar). The Ce-materials representative laterite minerals: Ce-doped oxyhydroxides k...

A variety of metabolic intermediates of the glycolytic pathway and tricarboxylic acid cycle have been found to be inhibitors of 6-phosphogluconate dehydrogenase isolated from sheep liver. All nucleoside 5’-triphosphates and 5’diphosphates are inhibitors with Ki values averaging 0.8 mM, nucleoside 5’-monophosphates are also inhibitory with Ki values averaging 6 mu. The 2’and 3’monophosphates of ...