Abstract Polyhydroxyalkanoates (PHAs) production from lignocellulosic biomass using mixed microbial cultures (MMC) is a potential cheap alternative for reducing the use of petroleum-based plastics. In this study, an MMC adapted to acidogenic effluent dark fermentation (DF) exhausted sugar beet cossettes (ESBC) has been tested in order determine its capability produce PHAs nine different synthet...

Abstract We show the ACIS trap maps developed for the CTI correction algorithm can be applied to the GRADED mode data. The technique uses the fltgrade information available in the GRADED mode telemetry, and average 3 × 3 PHAS images for each fltgrade pre-calibrated using the FAINT mode data. The results (recovery of photon energies and energy resolution) are nearly identical to those from the C...

Polyhydroxyalkanoates (PHAs) are aliphatic polyesters that biosynthesized and accumulate in bacterial cells. Among PHAs, poly(3-hydroxybutyrate-co‑3-hydroxyhexanoate)s [P(3HB‑co‑3HHx)s] known to be practical PHA copolymers with an appropriate degree of flexibility. In the biosynthesis PHA, ethanol induces chain transfer (CT) reaction, resulting low-molecular-weight PHA. this study, P(3HB‑co‑3HH...

The main objective of the present study is to assess and improve fermentation conditions produce polyhydroxyalkanoates (PHAs), such as homopolymer poly(3-hydroxybutyrate) (PHB) copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), from renewable inexpensive industrial waste by wild type Cupriavidus necator. Untreated residual glycerol, a byproduct generated biodiesel industry, was used...

In this work, delivery systems in the form of microparticles and films containing 1,2,4-trioxolane (ozonide, OZ) based on polyhydroxyalkanoates (PHAs) were developed. Main systems’ characteristics investigated: particle yield, average diameter, zeta potential, surface morphology, loading capacity, drug release profile microparticles, as well morphology profiles OZ-containing films. PHA-based OZ...

In this investigation, a sugarcane agroecosystem at a coastal tableland, in the northeast of Brazil, was screened to obtain bacteria strains able to synthesize poly-β-hydroxyalkanoates (PHA), using sucrose as the main carbon source. The potential to synthesize PHA was tested qualitatively by Sudan Black staining of colonies growing in different carbon sources: sucrose, glucose, fructose, propio...

In-depth interviews were conducted with 39 very religious people living with HIV (16 had ever and 23 had never discontinued antiretroviral therapy-ART) to assess the role of religion in these treatment decisions and in coping with HIV. Participants who had ever discontinued ART gave reasons such as: teachings and prophecies from religious leaders, and supporting Biblical scriptures all of which...

AIMS The aim of this study was to isolate a thermotolerant micro-organism that produces polyhydroxyalkanoates (PHAs) composed of medium-chain-length (mcl) HA units from a biodiesel fuel (BDF) by-product as a carbon source. METHODS AND RESULTS We successfully isolated a thermotolerant micro-organism, strain SG4502, capable to accumulate mcl-PHA from a BDF by-product as a carbon source at a cul...

Recombinant Escherichia coli harboring the medium-chain-length (MCL) polyhydroxyalkanoate (PHA) synthase gene has been shown to accumulate MCL-PHAs from fatty acids when FadB is inactive. However, the enzymes in fadB mutant E. coli responsible for channeling the beta-oxidation intermediates to PHA biosynthesis have not been fully elucidated. Only recently, two enzymes encoded by yfcX and maoC h...