It is well known that peripheral lymphocytes rarely divide in tissue culture; indeed, they have usually been considered to be the end cell in the pathway of differentiation. However, in the presence of certain mitogenic agents, of which phytohemagglutinin (PHA) is the best known example, a striking transformation occurs; the cells increase their over-all metabolic activity, enlarge, and divide....

BACKGROUND Polyhydroxyalkanoates (PHA) are synthesized by many bacteria in the cytoplasm as storage compounds for energy and carbon. The key enzymes for PHA biosynthesis are PHA polymerases, which catalyze the covalent linkage of 3-hydroxyacyl coenzymeA thioesters by transesterification with concomitant release of CoA. Pseudomonas putida GPo1 and many other Pseudomonas species contain two diffe...

Nhựa sinh học Polyhydroxyalkanoate (PHA) là loại nhựa do vi vật tổng hợp nên trong điều kiện dư thừa nguồn cacbon. Có nhiều có thể PHA, đó các loài chịu mặn như Bacillus, Halomonas... Tuy nhiên, thuộc vùng đảo Trường Sa chưa được quan tâm nghiên cứu. Nghiên cứu này trình bày một số kết quả phân lập và đánh giá khả năng PHA của chủng từ quần Sa. Trong 21 khuẩn TSLT21 tới 30,9% khối tế bào khô đế...

ownloade pose: This dose-escalation study evaluated the safety, tolerability, and pharmacokinetics (PK) of al Src inhibitor saracatinib (AZD0530) in patients with advanced solid malignancies. Tumor biopsy es were taken to investigate the effect of saracatinib on Src activity in tumors. erimental Design: Part A of the study followed a multiple-ascending dose design to establish the um tolerated ...

background: availability of antiretroviral therapies has transformed aids into a manageable chronic condition and im­proved well-being among people living with hiv/aids (pha) in developed countries. in developing countries however, such transformations are yet to occur due to socio-economic, systemic and environmental constraint. this study examined the impact of social, economic, psychological...

Amongst the polyhydroxyalkanoate (PHA) family, the class of medium-chain-length PHA ( mcl-PHA) that contains C6 to C14 monomers has attracted a lot of attention due to its extraordinary diversity. In particular, the possibility to adjust material properties by controlling the monomeric composition, and functionalize it via introduction of vinyl groups is of great interest1. Whereas short-chain-...

Assays were performed that measured both the rate and extent (or total capacity) of transfer RNA methylases in extracts of lymphocytes cultured in the presence and absence of phytohemagglutinin (PHA). The tRNA methylases of human peripheral blood lymphocytes undergoing blastogenesis in culture with PHA had a fiveto sixfold increase in rate and a threeto sevenfold increase in extent of methylati...

Polyhydroxyalkanoates (PHAs) have received considerable interest as renewable-resource-based, biodegradable, and biocompatible plastics with a wide range of potential applications. We have engineered the synthesis of PHA polymers composed of monomers ranging from 4 to 14 carbon atoms in either the cytosol or the peroxisome of Saccharomyces cerevisiae by harnessing intermediates of fatty acid me...

The emergence of resistance to imatinib (IM) mediated by mutations in the BCR-ABL domain has become a major challenge in the treatment of chronic myeloid leukemia (CML). Here, we report on studies performed with a novel small molecule inhibitor, PHA-739358, which selectively targets Bcr-Abl and Aurora kinases A to C. PHA-739358 exhibits strong antiproliferative and proapoptotic activity against...

We have studied cyclic adenosine 3',5'-monophosphate (cyclic AMP) concentrations in human peripheral blood lymphocytes after stimulation with phytohemagglutinin (PHA), isoproterenol, prostaglandins, and aminophylline. Purified lymphocytes were obtained by nylon fiber chromatography, and low speed centrifugation to remove platelets. Cyclic AMP levels were determined by a highly sensitive radioim...