Peroxins are proteins required for peroxisome assembly and are encoded by the PEX genes. Functional complementation of the oleic acid-nonutilizing strain mut1-1 of the yeast Yarrowia lipolytica has identified the novel gene, PEX24. PEX24 encodes Pex24p, a protein of 550 amino acids (61,100 Da). Pex24p is an integral membrane protein of peroxisomes that exhibits high sequence homology to two hyp...

space should have been devoted to describing the ex-age of cell biology. The book does not include sections periments that led to this information. For example, a on immunology/immunity, cancer, or development, section in Chapter 46 (box 46-1) contains a synopsis leaving these topics for more specialized texts, which I of key experiments in cell cycle research that I found personally find appro...

The development of new anti-Leishmania compounds and the study of the mode of action of existing drugs has long been hampered by the difficulties of obtaining sufficient numbers of intracellular amastigotes or to culture in the laboratory well-defined parasites representative of intracellular amastigote stages. Most of the early studies have been carried out on promastigotes, of which the metab...

Adult Refsum disease (ARD) is a progressive multisystem disorder that is characterized by retinitis pigmentosa, hearing and smell loss, skeletal deformities, and elevated levels of phytanic acid (PA) in tissues. Peripheral neuropathy, cardiac abnormalities, and skin ichthyosis are considered subsequent manifestations, and are often described in patients not treated with PA dietary restriction. ...

We identified a Saccharomyces cerevisiae peroxisomal membrane protein, Pexl3p, that is essential for protein import. A point mutation in the COOHterminal Src homology 3 (SH3) domain of Pexl3p inactivated the protein but did not affect its membrane targeting. A two-hybrid screen with the SH3 domain of Pexl3p identified Pex5p, a receptor for proteins with a type I peroxisomal targeting signal (PT...

P e x l l p (formerly Pmp27) has been implicated in peroxisomal proliferation (Erdmann, R., and G. Blobel. 1995. J. Cell Biol. 128: 509-523; Marshall, P.A., Y.I. Krimkevich, R.H. Lark, J.M. Dyer, M. Veenhuis, and J.M. Goodman. 1995. J. Cell Biol. 129: 345355). In its absence, peroxisomes in Saccharomyces cerevisiae fail to proliferate in response to oleic acid; instead, one or two large peroxis...