in order to study the effect of salicylic acid on antioxidant enzymes activity such as peroxidase (pod), gayacol peroxidase (gpx) and poly phenol oxidase (ppo), chlorophyll a, b and carotenoid in coriander, this study was done as completely randomized whit three replication in biocenter of zabol university, 2014. salicylic acid was in four level (0, 1, 1.5 and 2 mm). in comparison with control,...

BACKGROUND The alterations of the glutathione peroxidase enzyme complex system occur in physiological conditions such as aging and oxidative stress consequent to strenuous exercise. METHODS Authors optimize the spectrophotometric method to measure glutathione peroxidase activity in rat red blood cell membranes. RESULTS The optimization, when applied to age paired rats, both nulligravid and ...

Classical heme-containing plant peroxidases have been ascribed a wide variety of functional roles related to development, defense, lignification, and hormonal signaling. More than 40 peroxidase genes are now known in Arabidopsis thaliana for which functional association is complicated by a general lack of peroxidase substrate specificity. Computational analysis was performed on 30 near full-len...

The present study was set up to formulate a generally applicable schedule for the cellular demonstration of tumour index substances using routinely prepared histological material by a method which would also be reproducible and readable and allow simultaneous histological diagnosis. The carcinoembryonic antigen (Gold and Freedman, 1965) was used as the prime subject as high-quality reagents wer...

The kinetics of the oxidation of p-cresol by Compound II of horseradish peroxidase has been studied by the stopped flow technique at an ionic strength of 0.11 from pH 2 to 11. In acid solution the reaction is kinetically first order in jcresol, but in the alkaline region a saturation effect attributable to complex formation is observed. At very high pH an additional second order reaction betwee...

Eosinophil peroxidase has been implicated in promoting oxidative tissue damage in a variety of inflammatory conditions, including asthma. It uses H(2)O(2) to oxidize chloride, bromide and thiocyanate to their respective hypohalous acids. The aim of this study was to establish which oxidants eosinophil peroxidase produces under physiological conditions. By measuring rates of H(2)O(2) utilization...

The study of the stability enhancement of a peroxidase immobilized onto mesoporous silicon/silica microparticles is presented. Peroxidases tend to get inactivated in the presence of hydrogen peroxide, their essential co-substrate, following an auto-inactivation mechanism. In order to minimize this inactivation, a second protein was co-immobilized to act as an electron acceptor and thus increase...

Fungal secretory peroxidases mediate fundamental ecological functions in the conversion and degradation of plant biomass. Many of these enzymes have strong oxidizing activities towards aromatic compounds and are involved in the degradation of plant cell wall (lignin) and humus. They comprise three major groups: class II peroxidases (including lignin peroxidase, manganese peroxidase, versatile p...

A new method for the determination of glutathione peroxidase activity in erythrocytes was developed. The present method was applied to the measurement of hydrogen peroxide removal rates by glutathione peroxidase in erythrocytes at 70 microM hydrogen peroxide under simulated in vivo conditions. The removal rates by glutathione peroxidase in mouse erythrocytes were twenty-times faster than those ...

An indigenous wood rotting edible Lentinus kauffmanii isolated from Keeriparai forest of Western Ghats was studied for extracellular peroxidase production. Peroxidases are major extracellular lignolytic enzymes involved in lignocellulosic degradation. Physical factors such as pH, temperature and nutritional factors such as carbon, nitrogen sources and amino acids were standardized for the growt...