Endothelial cell leakiness is regulated by mediators such as thrombin, which promotes endothelial permeability, and anti-inflammatory agents, such as angiopoietin-1. Here we define a new pathway involved in thrombin-induced permeability that involves the atypical protein kinase C isoform, PKCzeta. Chemical inhibitor studies implicated the involvement of an atypical PKC isoform in thrombin-induc...

Tumor necrosis factor (TNF) has marked effects on permeability and procoagulant activity on tumor-associated neovasculature when used in isolation perfusion, the latter effect primarily mediated via induction of cell surface expression of tissue factor (TF) on endothelial tissue. However, the cellular events that result in rapid alterations in endothelial cell (EC) permeability after intravascu...

Pulmonary microvascular and alveolar epithelial permeability were evaluated in vivo by scintigraphic imaging during lung distension. A zone of alveolar flooding was made by instilling a solution containing 99mTc-albumin in a bronchus. Alveolar epithelial permeability was estimated from the rate at which this tracer left the lungs. Microvascular permeability was simultaneously estimated measurin...

Recent evidence has linked intestinal permeability to mucosal inflammation, but molecular studies are lacking. Candidate regulatory molecules localized within the tight junction (TJ) include Junctional Adhesion Molecule (JAM-A), which has been implicated in the regulation of barrier function and leukocyte migration. Thus, we analyzed the intestinal mucosa of JAM-A-deficient (JAM-A(-/-)) mice fo...

PURPOSE The aim of the study was the prediction of human intestinal permeability and fraction absorbed of oral dose using single-pass intestinal perfusion technique (SPIP) in rats. METHODS Permeability coefficients in anaesthetized rats were determined for 14 compounds. Drug solution in phosphate buffered saline (PBS) was perfused through a ingle-pass intestinal perfusion (SPIP) with flow rat...

Many studies have considered the effects of suspension properties on the dewatering process but fewhave focused on ore properties. Thus, the present work studied the effects of ore properties (density,particle size, mineralogy) on the dewatering process based on lab and pilot experiments. A hydrocyclonewas used to prepare the required samples for the experiments. To study the effects of mineral...

Epithelial tissues act as barriers between two fluid compartments, and the epithelial barrier function is provided by the epithelial cells and the tight junctions (TJs) that connect them. We have shown previously that chronic treatment of a cultured epithelial monolayer with phorbol ester tumor promoters induces an increase in transepithelial paracellular permeability and produces tumor-like po...

AIMS In this study, we wished to determine whether angiopoietin-1 (Ang1) modified the permeability coefficients of non-inflamed, intact continuous, and fenestrated microvessels in vivo and to elucidate the underlying cellular mechanisms. METHODS AND RESULTS Permeability coefficients were measured using the Landis-Michel technique (in frog and rat mesenteric microvessels) and an oncopressive p...

MORGAN, TREFOR, AND ROBERT MT. BERLINER. Permeability of the loop of Henle, uasu recta, and collecting duct to water, urea, and sodium. Am. J. Physiol. 215(l): 108-115. 1968.-The permeability of the loops of Henle, collecting ducts, and vasa recta have been studied by free-flow perfusion. When ADH was absent the diffusional permeability coefficient for THO in the descending limb (DL) was I 19 Z...

PURPOSE This study is to reveal the deformation of intervertebral disc (IVD), the stress distribution of solid phase and liquid phase, the variation of fluid flux and flow velocity in lumbar spine and the influence of different permeability parameters on them under intermittent compressive loading. METHODS A poroelastic FEM of L4-L5 is assigned with different permeability parameters to analyz...