A windowless flow cell has been developed for surface-enhanced Raman (SER) detection in liquid chromatography. Using colloidal silver as an active substrate, SER spectrometry of banned drugs in sport is presented. The experimental factors of primary influence on the analytical performance of the cell have been studied. The results of SER detection with the proposed cell have been compared to UV...

Background & Aims: In this study, using a liquid-liquid microextraction method for pre-concentration trace amounts of aflatoxins, the amount of Aflatoxins B1 and B2 in powdered milk was determined. Determination of aflatoxins was done by using high-performance liquid chromatography coupled with fluorescent detector. Method: Samples were extracted by immunoaffinity column (IAC) clean-up, and the...

Ion-pairing high performance liquid chromatography (IP-HPLC) was utilized to monitor the composition changes of blue gel pen ink entries on paper stored in different light conditions and natural environment. The chromatographic conditions were optimized by comparing the separation efficiencies of the blue gel pen inks using a series of ion-pairing reagents, including ammonium carbonate, ammoniu...

The coumarins: scopoletin, esculetin and fraxetin were isolated from the flowers of horse chestnut (Aesculus hippocastanum L., Hippocastanaceae) and identified by spectrophotometric methods (UV, 1H, 13C NMR, ESI-MS). Their content, determined using the Ultra Performance Liquid Chromatography (UPLC), was 0.41, 0.13 and 0.05%, respectively.

A solid phase extraction sorbent based on polyaniline/graphene nanocomposite is presented. The structure and morphology of synthesized nanocomposite were investigated by Fourier transform infrared spectroscopy, field emission scanning electron microscopy, X-ray diffraction and thermal gravimetric analysis. The dispersive solid phase extraction was employed to the isolation and preconcentration ...

the lack of authentic standards has limited the quantitative analysis of herbal drugs in biological samples. the present work demonstrated a practicable strategy for the assay of herbs and their metabolites independent of authentic standards. a liquid chromatography–electrospray ionization–mass spectrometry (lc–esi–ms) method for the qualitative and quantitative determination of the metabolites...