surfaces (Fig. 1). Most cells were not labelled and could be compared with controls. The morphology of the ThyI-labelled cells in culture was quite distinctive; they are the largest, with an eccentrically placed nucleus. Furthermore these cells do not accumulate I'H Iglycine, I 3H IGABA* or D-l )H laspartate as do other cells in the culture (see Beale & Osborne 1983). We therefore conclude that...

Ongole cattle in smallholder farms on Java, Indonesia, have been crossed with Limousin, which is not controlled repeatedly. The character of estrus affects the success Artificial Insemination. This study aimed to determine relationship between characters before double dose artificial insemination using frozen semen sexing. research was conducted Senggreng Village, Sumber Pucung District, Malang...

Objective: To develop methods for the isolation and purification of round spermatids (RS) from ejaculated semen specimens recovered from patients suffering from nonobstructive azoospermia for the purpose of performing intracytoplasmic round spermatid injections (ROSI) into human oocytes. Design: Prospective study. Setting: Audrology Institute of Lexington, Lexington, Kentucky. Patients: Six men...

a research plan was developed to evaluate the occurrence of enteroviruses, giardia cysts and cryptosporidium oocysts in thirty-five different sites in the city of mashhad, iran. the occurrence of these pathogenic microorganisms was evaluated to investigate a possible health risk in the communities. for the detection of enteroviruses in water samples, the conventional method of cell culture, usi...

Oxidative phosphorylation is a key process of intracellular energy transfer by which mitochondria produce ATP. Isolated mitochondria serve as a biological model for understanding the mitochondrial respiration control, effects of various biologically active substances, and pathophysiology of mitochondrial diseases. The aim of our study was to evaluate pig brain mitochondria as a proper biologica...

Chloroplasts, which perform photosynthesis, are one of the most important organelles in green plants and algae. Chloroplasts maintain an independent genome that includes important genes encoding their photosynthetic machinery and various housekeeping functions. Owing to its non-recombinant nature, low mutation rates, and uniparental inheritance, the chloroplast genome (plastome) can give insigh...

Type I and II methanotrophic bacteria can be cleanly separated by using Percoll buoyant density gradients. This simple and inexpensive method can be used to screen new isolates rapidly for preliminary classification. In addition, Percoll gradients can also be used to enhance standard enrichment techniques for methanotrophs from natural water samples.

Mitochondria from green leaves of spinach have been prepared using a three-step procedure involving differential centrifugation, partition in an aqueous dextran polyethylene glycol two-phase system and Percoll gradient centrifugation. The mitochondrial fractions after the different steps of purification were compared. The final mitochondrial preparation was totally free from chloroplast materia...