Cell penetrating peptides (CPPs) can be used as drug delivery systems for different therapeutic molecules. In this work two novel CPPs, pepR and pepM, designed from two domains of the dengue virus (DENV) capsid protein, were studied for their ability to deliver nucleic acids into cells as non-covalently bound cargo. Translocation studies were performed by confocal microscopy in HepG2, BHK and H...

The pseudopeptide backbone provided by N-(2-aminoethyl)-glycine oligomers with attached nucleobases has been widely utilized in peptide nucleic acids (PNAs) as DNA mimics. Here we demonstrate the suitability of this for formation structurally defined dye stacks. Toward goal a series merocyanine (PMC) connected to were prepared through synthesis. Our concentration-, temperature- and solvent-depe...

Abstract Amino acid decorated imidazole, pyrimidine and purine derivatives, resembling analogues of peptide nucleic acid's building blocks, have been synthesized by a three‐way multicomponent chemistry diaminomaleonitrile, one the most studied chemical precursors in prebiotic chemistry. The regioselectivity reaction between trimethyl orthoacetate selected panel α‐amino acids, was controlled ene...

The design of a non-viral gene delivery system that can release functional nucleic acid at the intracellular destination site is an exciting but also challenging proposition. ideal vector must be non-toxic, non-immunogenic, overcome extra- and intra-cellular barriers, protect cargo from degradation with stability over range temperatures. A new 15 amino linear peptide termed CHAT was designed in...

Here, we report the synthesis of 3,6,9-trioxaundecan-1-{4-[(2-Chloroethyl)Ethylamino)]-Benzylamino},11-Azide (CEBA). CEBA alkylates N7 guanine DNA thanks its chloroethyl group and can be coupled by a strain-promoted azide-alkyne cycloaddition to an alkynylated molecule. The optimization alkylation level pDNA reveals that expression encoded gene is preserved when it randomly modified with at mos...

Since the 1950s, reverse translation has been an enigmatic part of Crick’s central dogma molecular biology. It might be described as possibility to back-translate information from proteins nucleic acids (or codons). A few studies have attempted theorize and/or conduct in vitro experiments test likelihood translation, with ideas often involving creation peptide recognition sites that bridge and ...