•In silico screening finds 87 putative L-asparaginases from brackish Caspian Sea•Three candidate genes, cloned and expressed in E. coli, showed the desired activity•In coupled with functional validation bypasses cultivation bottleneck By 27,000 publicly available prokaryotic genomes, we recovered ca. 6300 type I 5200 II L-asparaginase highlighting vast potential of prokaryotes. water similar sa...

•DPF-3, a DPPIV family protease, protects C. elegans fertility•Loss of DPF-3 alters cellular endo-siRNA profiles and desilences transposons•DPF-3-mediated processing WAGO-1/-3 Argonaute proteins promotes siRNA sorting•A combination processing-refractive WAGO-1 WAGO-3 phenocopies loss Small RNA pathways defend the germlines animals against selfish genetic elements, yet pathway activities need to...

Interactions between proteins are fundamental for every biological process and especially important in cell signaling pathways. Biochemical techniques that evaluate these protein–protein interactions (PPIs), such as vitro pull downs coimmunoprecipitations, have become popular most laboratories essential to identify validate novel protein binding partners. Most PPIs occur through small domains o...

The prevalence of autoimmune diseases is on the rise globally. Currently, autoimmunity presents in over 100 different forms and affects around 9% world’s population. Current treatments available for are inadequate, expensive, tend to focus symptom management rather than cure. Clinical trials have shown that live helminthic therapy can decrease chronic inflammation associated with inflammatory b...

Age-related macular degeneration (AMD) is a leading cause of visual impairment. Anti–vascular endothelial growth factor drugs used to treat AMD carry the risk inducing subretinal fibrosis. We investigated use adrenomedullin (AM), vasoactive peptide, and its receptor activity-modifying protein 2, RAMP2, which regulate vascular homeostasis suppress The therapeutic potential AM-RAMP2 system was ev...

•Embryonic hypothalamic microglia are heterogeneous•A subpopulation of embryonic reside adjacent to NSCs•Maternal cold stress impacts signaling nearby NSCs in males•Exposure gestational leads disrupted social behaviors adulthood The interplay between neurons and as they integrate stressors regulate homeostasis is growing interest. We asked if the hypothalamus were likewise responsive and, so, w...

•Structure of native EspA adopts a unique fold compared with other T3SS tip proteins•Native different conformation from the chaperone-bound state•The structure reveals an electropositive pattern within its lumen•In vivo studies show that electrostatics play crucial role in function The type III secretion system (T3SS) is virulence mechanism employed by Gram-negative pathogens. forms proteinaceo...

This study was to investigate if endogenous pituitary adenylate cyclase-activating polypeptide (PACAP) can be released during direct splanchnic nerve stimulation in vivo, and to determine whether PACAP in the adrenal gland can modulate the medullary response to sympathoadrenal reflex. The output of adrenal catecholamine and PACAP38-like immunoreactivity (PACAP-38-ir) increased in a frequency-de...

Community shared bicyles Vélo’v: How to catch users with lasso Pierre Borgnat , ENS Lyon, CNRS, France Vélo’v is the community shared bicycle system in Lyon city. As a fully automated system, the uses of the bikes are all recorded and provide an exhaustive digital footprint of the moves done through the city with these bikes. As such, they can be used to reveal part of the activity of the city ...

Background: Protein glycosylation, the covalent attachment of carbohydrates, is very common, but in many cases the biological function of glycosylation is not well understood. Recently, uorescence energy transfer experiments have shown that glycosylation can strongly change the global conformational distributions of peptides. We intend to show the physical mechanism behind this structural e ect...