Autoimmune gastritis (AIG), a chronic inflammatory disease occurs as result of complex interaction between host-related and environmental factors. AIG may progress to severe atrophic secondary autoimmune-mediated parietal cell destruction in the stomach. can be diagnosed based on anti-parietal antibody tests endoscopy, which reveals widespread gastric corpus atrophy patients with low serum peps...

Atrophic gastritis (AG) is a well-recognized high-risk condition for developing gastric cancer (GC). Gastrin 17 (G17), a hormone secreted from antral G cells, regulates gastric acid secretion, and its serum level is a possible indicator of antral atrophy. Serum pepsinogen is well established as the indicator of AG involving the corpus. Here we investigated whether serum PG and G17 levels would ...

In the preceding communication, the molecular properties of pepsinogen in water and water-urea solutions were evaluated (1). In this report the rates of unfolding of pepsinogen as a function of pH and temperature in urea solutions are presented. A comparison can then be made between the influence of these variables on pepsinogen denaturation and the results of a similar study reported several y...

We investigated cholecystokinin (CCK) receptors on isolated gastric chief cells from guinea pig. CCK stimulated pepsinogen secretion from chief cells at the same efficacy as that induced by carbamylcholine. Binding of 125I-labeled CCK-33 (125I-CCK) to chief cells was temperature-dependent, and was saturable and reversible at 37 degrees C. Hofstee plots of the ability of CCK-8 to inhibit binding...

AIM To explore whether predisposition to autoimmune gastritis (AIG) is found in human leukocyte antigen (HLA), cytokine or killer cell immunoglobulin-like receptor (KIR) gene variations. METHODS Twelve Finnish patients with autoimmune-type severe atrophy of the gastric corpus were included. The patients' serum was analyzed for pepsinogen I and Helicobacter pylori (H. pylori) antibodies. DNA w...

BACKGROUND Gastric acid secretion in Japanese subjects decreases with aging. One of the possible causative mechanisms of this attenuated acid secretion is speculated to be a Helicobacter pylori induced chronic gastritis. The infection rate of this microorganism has decreased recently in Japan. AIMS To investigate whether gastric acid secretion has altered over the past 20 years, and if so, wh...

Casein kinase, which catalyzes the transfer of the terminal phosphate of ATP to dephosphorylated caseins, was prepared from homogenates of lactating bovine mammary tissue by differential centrifugation, followed by extraction of the 66,000 x g particulate fraction with Triton X-100. The enzyme required divalent cations and showed comparable activities with 15 mu Ca’+, 15 mu Mg2+, and 1 mu Mn2’....

Nonhydrolyzable guanine nucleotide analogues were used to evaluate the role of guanine nucleotide binding (G) proteins in regulating pepsinogen secretion from streptolysin O-permeabilized chief cells from guinea pig stomach. In the presence of 100 nM calcium, 100 microM guanosine 5'-(beta,gamma-imido)triphosphate or guanosine 5'-3-O-(thio)triphosphate (GTP gamma S) caused a 2- to 4-fold increas...

Although bile acids damage gastric mucosa, the mechanisms underlying tissue injury induced by these agents are not well understood. To determine whether bile acids alter gastric secretory function, we investigated the actions of sodium cholate, deoxycholate, lithocholate, and their taurine and glycine conjugates on a highly homogeneous population of gastric chief cells. Lithocholyltaurine (LCT)...