The serological and physicochemical properties of the following three forms of human anti-gamma-globulin factors were compared: (a) rheumatoid factors; (b) Milgrom type anti-gamma-globulin factors; and (c) factors directed against an antigen in human gammaG-globulin that is hidden in the intact molecule and revealed by enzymatic digestion at low pH. The property common to these factors is abili...

The susceptibility of ribonuclease to pepsin was demonstrated some time ago by Kunitz (1) and interesting conclusions about the structural basis of RNase activity have been derived from subsequent investigations by Anfinsen (2, 3). Since RNase is a single polypeptide chain (4) of 124 amino acids (5) held in a compact configuration largely through the restraint imposed by four disulfide bonds (4...

Antivenoms have been used successfully for more than a century and up to now constitute the only effective treatment for snakebites .The production of antivenin started long time ago when the calmette was prepared the antivenom in 1894.The method currently used to prepare antivenom by most of the manufacturers are originated from the method of Pope which was develop in 1938. Several new approac...

Normal adult rabbit corneas were digested with 5% pepsin and their collagens extracted with acetic acid. Collagen extracts were fractionated by differential salt precipitation. The 2.5 M NaCl fraction was then redissolved with tris buffer and precipitated with sodium acetate. The precipitate contained a high-molecular-weight disulfide-bonded aggregate which, upon reduction with mercaptoethanol,...

The aim of this study was preparation a soybean protein hydrolyzate with thrombin inhibitory activity and isolation, identification, characterization the active peptides from hydrolyzate. In study, inhibiting IC50 value 2.78 mg/mL prepared under improved pepsin hydrolysis condition, which exhibited highest in these protein-derived hydrolyzates. A novel nonapeptide FFPDIPKIK 2.36 mM screened by ...

In the last twenty years, elaborate models have been developed to formulate diets with greater accuracy in meeting the protein and amino acid requirements of lactating dairy cattle. Models for feeding protein to dairy cattle in the USA evolved from basic crude protein (NRC, 1978) to more complex systems based on rumen undegraded protein (RUP) and intestinal digestion of RUP (NRC, 2001). The NRC...

The relationship between the nature of in vitro digestion products and protein quality evaluation in rats was established. Eleven protein sources of animal and vegetable origin and of various purities were digested by pepsin then by pancreatin in a dialysis bag of 1,000 molecular weight cutoff. Animal proteins were generally better digested in vitro than vegetable proteins. Amino acid release p...

[14C]Taurocholate was orally administered to rats together with a definite amount of either casein- or soy protein-based diet and its postprandial movement along the digestive tract was investigated. A difference was observed between both dietary groups in intraluminal transit as well as mucosal accumulation of [14C]taurocholate in the ileum; namely the soy protein intake led to a decrease in t...

Human milk is known to contain several proteases, but little is known about whether these enzymes are active, which proteins they cleave, and their relative contribution to milk protein digestion in vivo. This study analyzed the mass spectrometry-identified protein fragments found in pooled human milk by comparing their cleavage sites with the enzyme specificity patterns of an array of enzymes....

In the hemoglobin method for the estimation of proteinase, denatured hemoglobin is digested under standard conditions, the undigested hemoglobin is precipitated with trichloracetic acid, and the amount of unprecipitated protein split products, which is a measure of the amount of proteinase present, is estimated with the phenol reagent which gives a blue color with tyrosine and tryptophane. Hemo...