BACKGROUND The use of lactic acid bacteria as vehicles to delivery antigens to immunize animals is a promising issue. When genetically modified, these bacteria can induce a specific local and systemic immune response against selected pathogens. Gastric acid and bile salts tolerance, production of antagonistic substances against pathogenic microorganisms, and adhesive ability to gut epithelium a...

要 旨 単 純 ヘ ル ペ ス ウ イ ル ス1型(HSV-1)お よ び2型(HSV-2)の 遺 伝 子 を 同時 に検 出 す るmultiplex real-time PCR法 を 開発 し た. HSV-1お よびHSV-2のgpD領 域 を標 的 と し,プ ラ イ マ ー お よ びTaqMan蛍 光 プ ロ ー ブ を 独 自 に 設 計 し た.検 出感 度 は15copies/tubeで,HSV-1お よ びHSV-2と も15か ら1.5×106 copies/tubeの 範 囲 で 定 量 が 可 能 で あ っ た.real-time PCR法 はPCR法 よ り も103倍 感 度 が 高 く,他 の ヘ ルペ ス属(CMV, EBV, HHV6, HHV-7お よ びVZV) DNAは 検 出 され なか っ た. 以 上 の結 果 か ら,今 回 開 発...

In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes and antimicrobial resistance testing w...

The present study investigated and identified genetic diversity between native White chicken lines two commercial Broiler (Rose) Layer (Isa Brown) breeds using RAPD markers a sequencing technique. All primers applied produced 151 scorable bands with percentage polymorphic loci of 54.93 % within populations, as per the results marker. maximum amplified fragment by primer OPC-11 was 22 fewest OPA...

Abstract The migration of parents or children may bring risks to children’s academic performance, but intergenerational effects on the performance migrant workers’ have been underexplored. This study aims investigate how grandparents’ socioeconomic status (G1) and parents’ proximal distal (G2) influence (G3) corresponding impacts left-behind children. data used in this were collected from a sur...

Campylobacter jejuni is now recognized as one of the main causes of bacterial foodborne disease in the Czech republic with Campylobacter coli less frequently implicated. Foods of animal origin, in particular poulty, have been identified as significant sources of this enteropathogen as a result of infection and contamination at the pre-harvest and harvest levels. In the period from March 2005 to...

OBJECTIVE Estimate the seroprevalence of influenza A virus in various commercial poultry farms and evaluate specific risk factors as well as analyze their genetic nature using molecular assays. MATERIALS AND METHODS This report summarizes the findings of a national survey realized from October 2010 to May 2011 on 800 flocks in 20 governorates. Serum samples were screened for the presence of s...