Objectives: The symbiosis of Trichomonas vaginalis and Mycoplasma hominis is the first described association between two obligate human parasites. Trichomonas is the niche and the vector for the transmission of M. hominis infection. This clinically significant symbiosis may affect T. vaginalis virulence and susceptibility to treatment. The aims of this study were to investigate the intracellula...

Thirty-six synovial fluid samples of temporomandibular joints were obtained from 33 patients with pain and anterior disk displacement (closed lock) in the joints. DNAs were prepared from the samples and amplified by a PCR-based assay specific for Mycoplasma salivarium or Mycoplasma fermentans. Of the 36 samples, five (14%), three (8%), and 19 (53%) were positive for M. salivarium, M. fermentans...

Mycoplasma pneumoniae is a particularly important pathogen that causes community acquired pneumonia in children. In this study, a rapid test was developed to diagnose M. pneumoniae by using a colloidal gold-based immuno-chromatographic assay which targets a region of the P1 gene. 302 specimens were analyzed by the colloidal gold assay in parallel with real-time PCR. Interestingly, the colloidal...

We studied the effectivity of a commercial antibiotic kit Mycokill AB for the elimination of mycoplasma contamination in virus strains. The contaminated virus strains were first filtered, treated with Mycokill AB for three hours and repeatedly passaged in its presence in the cultivation medium in pure cell lines. Three passages in the presence of Mycokill AB were invariably followed by three pa...

A 39-year-old Philipino man presented with acute onset fever and headache. Neurological examination was normal except for neck stiffness. There was no history of chest pain, cough or breathlessness. Cerebrospinal fluid (CSF) showed a mild increase in protein with normal sugar and lymphocytic pleocytosis. CSF PCR for herpes simplex and varicella zoster virus was negative. He developed acute righ...

mycoplasma ovipneumoniae is an important respiratory pathogen of sheep across the world. in the present study nasal swab samples (n=778) were processed and 51isolates of m. ovipneumoniae were identified through biochemical tests and pcr. difference in the isolation rate among districts (n=12), age groups (n=3), breads (n=6) was assessed. similarly the isolate rate among healthy (n=513) and resp...

The performances of five commercial TaqMan real-time PCR assays for the detection of Mycoplasma pneumoniae in respiratory tract specimens were evaluated in comparison with an in-house real-time PCR. All kits allowed prompt and specific results, validated by the use of an internal control. The Nanogen kit showed the best clinical sensitivity.

Respiratory culture and multiplex PCR for Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, and Chlamydophila pneumoniae were applied to sputum, nasopharyngeal swabs, and nasopharyngeal aspirates from 235 adult patients with community-acquired pneumonia and 113 controls. Both culture and multiplex PCR performed well with the different samples and appear to be useful as di...

An investigation was done on outbreak of acute respiratory disease in goats in Central zone of Tanzania, the investigation involved 3 districts. (Mpwapwa, Dodoma and Kilosa districts). The study involved goats from the field, livestock markets and abattoirs. Samples from clinical and pathological examination of goats with respiratory distress were subjected to microbiological and molecular anal...

one of the main problems in cell culture is mycoplasma infection. it can extensively affect cell physiology and metabolism. as the applications of cell culture increase in research, industrial production and cell therapy, more concerns about mycoplasma contamination and detection will arise. this review will provide valuable information about: 1. the ways in which cells are contaminated and the...