Background: The aim of this study was to determine the prevalence of virulence-associated genes and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) analysis of Campylobacter spp. isolated from children with diarrhea in Iran. Methods: A total of 200 stool specimens were obtained from children under 5 years during July 2012 to July 2013. Detection of C. jejuni and C. coli was perfo...

  Herpes simplex virus type 1 establishes a latent infection in the peripheral nervous system following primary infection. During latent infection, virus genome exhibit limited transcription, with the HSV LATs consistently detected in latency infected ganaglia. Following ocular infection viral latency develops in the trigeminal ganglia. In this study PCR has been used for detection of HSV-1 nuc...

Quantitative PCR (Q-PCR or real-time PCR) approaches are now widely applied in microbial ecology to quantify the abundance and expression of taxonomic and functional gene markers within the environment. Q-PCR-based analyses combine 'traditional' end-point detection PCR with fluorescent detection technologies to record the accumulation of amplicons in 'real time' during each cycle of the PCR amp...

The aim of this study was to compare conventional 16S rRNA gene PCR, real-time 16S rRNA gene PCR and real-time Mycoplasma genitalium adhesin protein (MgPa) gene PCR as detection methods for M. genitalium infection. The study also determined the prevalence of M. genitalium in male and female patients attending a sexually transmitted infections clinic in a rural area in the west of Sweden. First ...

Rabies are one of the major zoonoses in Mali. The control this disease is based on use effective diagnostic techniques, management human cases after exposure and medical prophylaxis animals. Diagnosis usually made by detection viral antigen direct immunofluorescence (IF). Due to increasing variability genus Lyssavirus observed, it was found necessary a complementary technique, namely reverse tr...

Abstract Koi herpesvirus (KHV) has been responsible for worldwide KHV disease (KHVD) outbreaks, resulting in high mortality rates, and reported Cyprinus carpio cultivation. A Triplex Polymerase Chain Reaction (T-PCR) was developed the detection of gene to improve diagnosis infection. Three sets primers were designed targeting specific sequences which frequently used PCR assay detecting (modific...

to determine the role of ddx3y gene in spermatogenesis and infertility in bulls, blood samples were collected from five infertile bulls (azoospermic; no sperm in the semen) at the animal breeding center in karaj, iran. the recommended human primers by eaa/eqmn were investigated using the blastn database for sts marker detection. alignment of sts marker genes with bovine genome was performed. pr...

the clar of streptomyces clavuligerus in the clavulanic acid gene cluster encodes a transcriptional regulator that controls clavulanic acid biosynthesis. the main goal of this study was isolation and molecular detection of the clar gene and its cloning in the streptomyces specific vector (pma:: hyg). by cinsideration of the clar gene’s start codon, the specific primers were designed. after geno...