a 9 bp deletion between cytochrome oxidase subunit ii and trna lys genes in mitochondrial dna, has proven to be an extremely informative marker for tracing population history. using the pcr-page and pcr-sscp techniques in this study, 152 dna samples collected from iranian populations were screened for the 9 bp deletion. no deletion was observed. a length polymorphism, most probably a 9 bp tripl...

Lentiviruses are potentially advantageous compared to oncoretroviruses as gene transfer agents because they can infect nondividing cells. We demonstrate here that human immunodeficiency virus type 1 (HIV-1)-based vectors were highly efficient in transducing purified human hematopoietic stem cells. Transduction rates, measured by marker gene expression or by PCR of the integrated provirus, excee...

Laser microdissection (LM) provides a useful method for isolating specific cells or tissues from biological samples. Here, we adapted microdissection protocols to allow high-resolution transcript analysis of different tissues from developing Arabidopsis seed. Sufficient RNA ( approximately 50 ng) was extracted from endosperm tissue for RT-PCR. However, to obtain enough RNA for microarray analys...

The aim of the present work was to evaluate the prevalence and efficacy of IBR control measures in selected cattle herds in Lithuania. Serological investigations in 1997 2007 years (34600 blood sera samples tested) showed that BHV-1 infection mostly detected in cows (33.86%), less in heifers (8.77%) and in bulls group (1.69%). In majority of the cases seropositive bulls were detected in quarant...

The human-specific HF183 Bacteriodes 16S rRNA genetic marker can be used to detect human faecal pollution in water environments. However, there is currently no method to quantify the prevalence of this marker in environmental samples. We developed a real-time polymerase chain reaction (PCR) assay using SYBR Green I detection to quantify this marker in faecal and environmental samples. To decrea...

The Polima system of cytoplasmic male sterility (Pol CMS) was widely used for hybrid seed production, but with high probability occurrence maternal false due to the fertility CMS line greatly affected by ambient temperature, therefore, detection hybrids is key purity identification. In present study, SSR markers were designed based on flanking sequences Pol restorer gene  Rfp , and applica...

BACKGROUND AND OBJECTIVE Aggregatibacter actinomycetemcomitans is involved in oral and systemic infections, and is associated with, eg aggressive forms of periodontitis and with endocarditis. The cagE gene encodes a ≈39 kDa putative exotoxin expressed by A. actinomycetemcomitans. The level of conservation of cagE, and its possible significance in periodontal disease, has not yet been thoroughly...

Y chromosomal microdeletions at the azoospermia factor locus and chromosome abnormalities have been implicated as the major causes of idiopathic male infertility. A marker chromosome is a structurally abnormal chromosome in which no part can be identified by cytogenetics. In this study, to identify the origin of the marker chromosomes and to perform a genetic diagnosis of patients with azoosper...

To detect integration of a transgene in genetically modified plants that have a native copy of the gene, a simple and reliable PCR-based method has been established. This method is then used to screen for transgenic wheat plants that have been transformed with the high molecular weight glutenin subunit Dy10 gene. Two PCR primers were designed, the forward one matched upstream flanking nucleotid...

Based on protein polymorphism and results obtained with RAPD-PCR and ISSR-PCR methods, the domestic and wild Artiodactyla and Perissodactyla (14 and 7 species, respectively) were compared. The marker-specific species differentiation in domestic and wild species was observed, leading to the hypothesis of the “subgenome” existing in domestic species. It is assumed that “subgenome” contains certai...