We evaluated the frequency of translocation renal cell carcinoma (RCC) by reverse transcription polymerase chain reaction (RT-PCR) and how well the TFE3 immunoreactivity is concordant with TFE3 gene translocation status proved by fluorescence in situ hybridization (FISH) assay and RT-PCR. TFE3 and Cathepsin K expression was analyzed by immunohistochemistry in 185 RCC cases, and 48 cases either ...

INTRODUCTION In this study, we determined: (1) the utility of an untimed sample of urine protein/creatinine ratio (PCR) as a screening test for proteinuria, (2) its ability to accurately measure proteinuria, and (3) cutoff values for PCR predicting protein content in a 24-h urine collection sample (24hP) of 0.5, 1.0, and 2.0 g/day. METHODS Analysis was performed on data from a single lupus co...

Genotyping methods are essential to understand the transmission dynamics of Acinetobacter baumannii. We examined the representative genotypes of A. baumannii at different time periods in select locations in Ohio, using two rapid automated typing methods: PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS), a form of multi-locus sequence typing (MLST), and repetitive-sequence...

Abstract Patients with locally advanced and potentially curable esophageal cancer (EC) should receive trimodality treatment, which involves neoadjuvant concurrent chemoradiotherapy (nCRT) followed by esophagectomy. The maximum standard uptake value (SUVmax) in SUV of 18FDG-PET/CT have been reported to be useful predicting the prognosis EC patients treated nCRT subsequent We aimed determine path...

Objective: To describe and molecularly characterize an outbreak of dengue virus (DENV) infection in Cajamarca, Andean region Peru.Methods: A total 359 serum samples from patients with acute febrile illness were assessed for the presence DENV via RT-PCR, ELISA NS1, IgM IgG Peru January 2017 to June 2017. The evaluation different diagnostic tests their applicability was performed.Results: Dengue ...

Swinepox virus (SWPV) is the only member of Suipoxvirus genus Poxviridae family and an etiologic agent a worldwide disease specific for domestic wild pigs. SWPV outbreaks are sporadically recorded in different regions Russia. In 2013, outbreak causing skin lesions was registered on pig farm The presence scab samples assessed by in-house real-time PCR, reference PCR amplification, nucleotide seq...

Teknologi 3D printing atau yang umum disebut sebagai additive manufacturing merupakan salah satu metode rapid prototyping saat ini semakin luas digunakan. Acrylonitrile butadiene styrene (ABS) jenis material penggunaannya cukup masif dalam teknologi printing. Penelitian menggunakan desain eksperimen orthogonal arrays L9 ( ). Adapun parameter proses digunakan adalah temperature nozzle, base plat...

INTRODUCTION Polymerase chain reaction (PCR)-based molecular techniques are useful adjunctive tools in the diagnosis of cutaneous T-cell lymphomas (CTCL). This study compares the sensitivity of PCR analysis of the T-cell receptor-gamma (TCR-gamma) gene rearrangements using conventional polyacrylamide gel electrophoresis (PCR-PAGE) and fluorescent capillary electrophoresis (PCR-FCE). MATERIALS...