[this article has been retracted for duplicate publication] background: the pathological classification of cryptogenic fibrosing alveolitis has been a matter of debate and controversy for histopathologists. objective: to identify and specify the glycotypes of capillary endothelial cells in usual interstitial pneumonia (uip) compared to those found in normal tissue. methods: sections of formalin...

osteosarcoma is a relatively uncommon malignancy however, it is the most common form of primary malignant bone tumors in human. diagnosis and prognosis of patients with osteosarcoma is limited to clinico-radiopathological parameters, whereas molecular markers of tumor aggression have been poorly identified. survivin, an inhibitor of apoptosis (iap), is unique for its expression in human tumors ...

Understanding the role of factors that regulate intestinal epithelial homeostasis and response to injury and regeneration is important. The current literature describes several different methodological approaches to obtain images of intestinal tissues for data validation. In this paper, we delineate a common protocol relating to the derivation and processing of mouse intestinal tissues. Proper ...

Programmed death 1 (PD-1) protein and its ligands, PD-L1 are two proteins that have been widely studied in many solid tumours. However, only limited observations were made lymphomas, which mainly Hodgkin lymphomas. We aimed to evaluate the expressions of PD-1 tumour microenvironment cells primary extranodal non-Hodgkin lymphoma (peNHL). A cross-sectional study using 87 archived formalin-fixed p...

The feasibility was studied of in situ hybridisation using chromosome specific DNA probes on paraffin wax embedded normal and malignant tissues from different organs. Both isolated nuclei and 5 microns sections were used in in situ hybridisation experiments with biotinylated repetitive DNA probes specific for the centromeric regions of chromosomes 1 and 17. The hybridisation results were visual...

Title of dissertation: PROTEOME ANALYSIS OF FORMALIN-FIXED AND PARAFFINEMBEDDED TISSUE Tong Guo, Doctor of Philosophy, 2008 Dissertation directed by: Professor Cheng S. Lee Department of Chemistry and Biochemistry Because of the long history of the use of formalin as the standard fixative for tissue processing in histopathology, these archival formalin-fixed and paraffinembedded (FFPE) tissues ...

Objective: The aim was to detect the expression of PR and CD146 in paraffin-embedded tissue sections of endometrioid adenocarcinoma by using QDs double-labeling immunofluorescence, and evaluate the applied value of QDs double-labeling immunofluorescence in endometrioid adenocarcinoma. Methods: To detect the expression of PR and CD146 on 140 cases of paraffin-embedded tissue sections of endometr...

Tissue microarrays (TMAs) are widely used to analyze gene expression in multiple pathological samples on a single slide. Currently, most TMA slides are made by coring paraffin-embedded tissues and arraying them into a paraffin block, from which TMA sections are cut. However paraffin-based TMA technology may not be compatible with frozen clinical tissue samples, which have a higher quality of RN...

INTRODUCTION Literature controversies exist regarding the prognostic value of VHL mutations. The objective was to compare paraffin-embedded and frozen section specimens for VHL mutations detection and to evaluate the reliability of DNA analysis in formalin-fixed tissues. METHODS Seventy-six patients with clear cell renal cell carcinoma (RCC) previously assessed for VHL status from frozen samp...