Background: TRR14 protein is a small member of a multi-gene family in Arabidopsis and is the first ones found during screening of seedlings for their resistant to the trehalose sugar.Objectives: Characterization ofTRR14 over-expressed plants with respect to morphological changes, growth and photosynthesis related parameters.Materials and methods: TRR14gene was isolated from Arabidop...

The study aims to produce Expansin protein isolated from a young tomato plant by using Escherichia coli which is used in recombinant production. Continuous culture the most common method grow cells for In study, K12 strain of E. was as production protein. LeExp1 gene plant. Since related found very small amounts plants, it has been reproduced PCR and made workable with this method. T17 vector (...

Somaclonal variation is one of the possible sources of variation in plant breeding. To evaluate the usefulness of somaclonal variation for creating variation in Iranian cultivars of wheat, proteins, enzymes and chromosome number of regenerated and seed-produced plants of wheat (Alamout, Hyrmand and Maroon) were studied. Plantlets obtained from the immature embryos and seed culture were collecte...

INTRODUCTION SDS-polyacrylamide gel electrophoresis (SDS-PAGE) represents the second-dimension separation of two-dimensional (2D)-PAGE. Large-scale proteome analysis usually requires simultaneous electrophoresis of batches of second-dimension SDS-PAGE gels to maximize the reproducibility of 2D electrophoresis protein profiles. This requirement is most easily met using multiple, vertical second-...

Background: Despite demonstration of molecular and biochemical changes induced by heat shock on Leishmania, the immunological importance of such changes has not been elucidated.  Objective: Studying the effect of two excretory factors prepared under heat shock and ambient temperature from Leishmania major on Balb/c splenocytes function.  Methods: The parasites were cultured at 25°C and then sub...

Background: Herpes simplex virus type 2 (HSV-2) is highly prevalent and major cause of genital herpes in humans. The life-long nature of infection and the increasing prevalence of genital herpes imply that vaccination is the best strategy for controlling the spread of infection and limiting HSV disease. HSV glycoprotein D (gD) is one of the most important viral immunogen which has an essential ...

Insulin-like growth factor 1 (IGF-1) is a polypeptide hormone produced mainly by the liver in response to the endocrine growth hormone (GH) stimulus. This protein is involved in a wide range of cellular functions, including cellular differentiation, transformation, apoptosis suppression, migration and cell-cycle progression and other metabolic processes. In the current study, human heart cDNA w...