To characterize the sites in human p53 that become phosphorylated in response to DNA damage, we have developed polyclonal antibodies that recognize p53 only when it is phosphorylated at specific sites. Several attempts to generate an antibody to p53 phosphorylated at Ser(6) using a phosphoserine-containing peptide as an immunogen were unsuccessful; however, phosphorylation-specific antibodies w...

DNA polymerases are versatile tools used in numerous important molecular biological core technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome sequencing, and nucleic acid based diagnostics. Taking into account the multiple DNA amplification techniques in use, different DNA polymerases must be optimized for each type of application. One of the current tendencies...

Two clones, CA4-2 and CA5-4, which produced agglutinating monoclonal immunoglobulin M (IgM) antibodies (MAbs) against mannan antigens of Candida albicans serotype A, were established. The specificity of each MAb was determined by slide agglutination tests for cross-reactivity patterns against the homologous and six other strains of Candida and a strain of Torulopsis: C. albicans serotype B, C. ...

EFHC1 gene mutations have been described in patients with juvenile myoclonic epilepsy (JME) and other types of idiopathic generalized epilepsy. We generated Efhc1-defi cient mouse and found that the mouse showed spontaneous myoclonus and increased susceptibility to a convulsant, pentylenetetrazol, further supported and confi rmed that EFHC1 is the gene for JME. Myoclonin1 protein encoded by EFH...

Abstract Background: Thalassemias are the most common inherited blood disorders caused by some mutations which can reduce the synthesis of globin chains. Iron overload and its organ deposition are responsible for functional abnormalities and tissue injury in patients who affected by β-thalassemia major. The aim of this case-control study was evaluation of hematological parameters, oxi...

According to the literature, p-aminobenzoic acid (PAB) is excreted in the urine mostly as the free acid and its N-acetyl derivative; the remaining part is eliminated conjugated with glucuronic acid and as p-aminohippuric acid (PAH) (Ellinger & Hensel, 1914'; Hensel, 1914; Cohen & McGilvery, 1946; Salassa, Bollman & Dry, 1948). There is no agreement concerning the formation of PAH. Thus, Beyer, ...

Background: This study evaluated the nutritional status, prooxidant-antioxidant balance (PAB) and the effect of hospitalization on these factors in patients with myocardial infarction (MI).Methods: Totally 57 patients diagnosed with MI were recruited (mean age of 58.44±12.80). Nutritional status was assessed using the Nutritional Risk Screening (NRS-2002) questionnaire in patients admitted to t...

While numerous researchers have investigated group behavior of robots which are each controlled in a behavior-based manner, none have yet thoroughly investigated the possibilities of extending the port-arbitrated behavior (PAB) paradigm across networks of robots. We present an extension to the well-deened PAB techniques of behavioral interaction which provides standard abstractions for nessagin...

We describe the first application in Greece of a telemetrically adjustable device for pulmonary artery banding (FloWatch-PAB device) in a 2-month-old baby, weighing 3.6 kg, with congenital heart disease (complete atrioventricular septal defect with pulmonary hypertension). The surgical application of the device was easy, the postoperative course of the patient was smooth, and the telemetric reg...

Photoexcitation experiments performed in oxygen deficient but metallic YBa,Cu,O, c-axis oriented films revealed that the persistent photoinduced enhancement of conductivity and superconductivity are not restricted to the insulating side of the metal-insulator transition. A clear increase of the critical temperature T,, which is accompanied by a decrease of the resistivity in the normal state pa...