Single dilution ELISAs were standardised for the determination of antibody titres against Theileria annulata using three antigens namely soluble piroplasm, cellular schizont or soluble schizont antigens. Antibody titres of 20 cattle serum samples of known identity were determined by multi-dilution ELISA using the three antigens. The ratio of the optical density (OD) of known positive and known ...

Background: Pneumococcal vaccination is recommended to patients with rheumatoid arthritis (RA) and primary Sjögren’s syndrome (pSS). However, little is known whether the diseases influence pneumococcal vaccine response. This study aimed to investigate antibody response and functionality of antibodies following immunization with 13-valent pneumococcal conjugate vaccine (PCV13) in RA patients or ...

AIM OF THE STUDY Podocalyxin-like 1 is a transmembrane glyco-protein whose overexpression associates in many cancers with poor prognosis and unfavorable clinicopathological characteristics. Until now, its prognostic value has never been studied in pancreatic ductal adenocarcinoma (PDAC). The aim of this study was to investigate podocalyxin expression in PDAC by a novel monoclonal antibody and a...

HIV infected individuals in malaria endemic areas experience more frequent and severe malaria episodes compared to non HIV infected. This clinical observation has been linked to a deficiency in antibody responses to Plasmodium falciparum antigens; however, prior studies have only focused on the antibody response to <0.5% of P. falciparum proteins. To obtain a broader and less-biased view of the...

BACKGROUND Systemic Lupus Erythematosus (SLE) is a chronic autoimmune disease which is characterized by B-cell abnormality and auto-antibody generation. Since bacterial infections are the most important causes of mortality in these patients, pneumococcal vaccination is recommended for children with SLE. OBJECTIVE To investigate humoral immunity and specific-antibody formation in response to a...

Septicemic Epizootica (SE) in livestock caused by bacterial infection of Pasteurella multocida. The serotype P. multocida Indonesia is the Asian B:2. objective this study to determine antibody responses cattle after vaccinating with local isolates Local isolate SE Vaccine. Ten female aged around one year used experiment. Cattle divided into 3 groups, Group A (n=4) vaccinated vaccines, B commerc...

the aim of this study was to investigate the hemagglutination properties of caprine herpesvirus-1(cphv-1) and their application for cphv-1 antigen detection and evaluation of the dynamics of postinfectious/vaccine antibody production. cphv-1 hemagglutination activity was proved only with mouseerythrocytes, pbs and saline solutions at 4°c and 22°c. in vaccinated animals, goats and bucks withresp...

OBJECTIVE The aim of this study is to evaluate the clinical manifestation and prognostic factors of anti-melanoma differentiation-associated gene 5 (MDA5) antibody-associated interstitial lung disease (ILD) with DM. METHODS Fourteen patients who presented with anti-MDA5 antibody and 10 patients with anti-aminoacyl-tRNA synthetase (ARS) antibody were enrolled. All patients were diagnosed as ha...

Recently, using polymerase chain reaction (PCR) technique, the DNA of cytomegalovirus was detected from depigmented white patches in patients with vitiligo. The reactivation of infection and occasional anti-CMV IgM circulating among patients infected by cytomegalovirus has been reported for years. We have studied 26 patients with clinical signs and symptoms of vitiligo, some of whom had a ...