The results of a comparative study of the respiration rates of mitochondria in saponin-skinned rat cardiac fibers (SF) and in fibers treated with saponin and collagenase (SCF) suggest that only about half of the whole population of mitochondria manifest their activity in SF, in contrast to SCF, in response to extracellular substrates of oxidative phosphorylation. The apparent K(m) value for ADP...

The article presents the results of an in vitro study synergetic effect evaluation combined preparation based on coordination complex ethylmethylhydroxypyridine succinate and trimethylhydrazinium propionate energy metabolism cell respiration. aim was to evaluate mitochondria-directed action metabolic antioxidant succinic acid with relation optimizing cells under oxidative stress conditions, as ...

In current studies in this laboratory on the composition and activity of plant mitochondria as a function of growth, it has been noted that well washed mitochondrial preparations contain about 1 per cent of the dry weight as ribonucleic acid. Most of this RNA may be due to microsomal contamination, for electron micrographs show corn mitochondrial preparations to be quite heterogeneous (1). Howe...

Background: At sites of inflammation, monocytes carry out specific immunological functions while facing challenging bioenergetic restrictions. Objectives: Here, we investigated the potential human to adapt under conditions reduced energy supply by gradually inhibiting oxidative phosphorylation (OXPHOS) glucose free conditions. Methods: We modelled this with myxothiazol, an inhibitor mitochondri...

The present investigation was undertaken in order to evaluate the contributions of ATP synthesis and proton leak reactions to the rate of active respiration of liver mitochondria, which is altered following dexamethasone treatment (1.5 mg/kg per day for 5 days). We applied top-down metabolic control analysis and its extension, elasticity analysis, to gain insight into the mechanisms of glucocor...

Changes in the kinetics and regulation of oxidative phosphorylation were characterized in isolated rat liver mitochondria after 2 months of ethanol consumption. Mitochondrial energy metabolism was conceptually divided into three groups of reactions, either producing protonmotive force (Deltap) (the respiratory subsystem) or consuming it (the phosphorylation subsystem and the proton leak). Manif...

Mitochondrial dysfunction and oxidative stress are strongly implicated in neurodegenerative diseases and epilepsy. Strikingly, neurodegenerative diseases show regional specificity in vulnerability and follow distinct patterns of neuronal loss. A challenge is to understand, why mitochondria fail in particular brain regions under specific pathological conditions. A potential explanation could be ...

Territo, Paul R., Vamsi K. Mootha, Stephanie A. French, and Robert S. Balaban. Ca21 activation of heart mitochondrial oxidative phosphorylation: role of the F0/F1ATPase. Am. J. Physiol. Cell Physiol. 278: C423–C435, 2000.—Ca21 has been postulated as a cytosolic second messenger in the regulation of cardiac oxidative phosphorylation. This hypothesis draws support from the well-known effects of C...

Mitochondrial dysfunction causes numerous human diseases and is widely believed to be involved in aging. However, mechanisms through which compromised mitochondrial gene expression elicits the reported variety of cellular defects remain unclear. The amino-terminal domain (ATD) of yeast mitochondrial RNA polymerase is required to couple transcription to translation during expression of mitochond...