Tumor necrosis factor α (TNF-α) receptor-associated factor 2 (TRAF2) regulates activation of the c-Jun N-terminal kinase (JNK)/c-Jun and the inhibitor of κB kinase (IKK)/nuclear factor κB (NF-κB) signaling cascades in response to TNF-α stimulation. Gene knockout studies have revealed that TRAF2 inhibits TNF-α-induced cell death but promotes oxidative stress-induced apoptosis. Here we report tha...

Introduction: Increased oxidative stress is widely accepted to be a factor in the development and progression of Alzheimer’s disease. Triazine derivatives possess a wide range of pharmacological activities including anti-oxidative and anti-in.ammatory actions. In this study, we aimed to investigate the possible protective effect of 3-thioethyl-5,6-dimethoxyphenyl-1,2,4-triazine (TEDMT) on H2O2-...

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Chlorophyllins inhibit oxidative phosphorylation in rat liver mitochondria, a-ketoglutarate oxi­ dation is more sensitive than oxidizing succinate or /?-hydroxybutyrate oxidation. When chlorophyllin is present, a sudden illumination of mitochondria incubated in the dark leads to a remarkable decrease of their 0 2-comsumption. In the absence of light the inhibition is diminished but not abolished.

2,4-Dinitrophenol fails to stimulate the breakdown of the soluble high energy intermediate of oxidative phosphorylation isolated from Alcaligenes faecalis extracts. Once the intermediate is broken down, by other reactions, the coupling enzyme is prevented from reassociation with the electron transport particles by 2,4-dinitrophenol. Phosphorylating particles washed with 2,4-dinitrophenol lose c...

2.5. hloore, A. I,., I>ay, 1). A,, Dry, 1. H. and Wiskich, J. 7’. (1000) in [Highlights in IJbiquinone Research (I,enaz, G.9 Ilarnebei, O., Rabbi. A. and Hattino, M., eds.), pp. 170173. Taylor and Francis, London 2(1. tiroger, A. ;und tilingenberg, M. ( 1 973) Eur. J. Hiochem. 24, 358-368 27. Dry, I. R. Moore. A. I,.. Day, I). A. and Wiskich, J. T. ( I O X O ) Arch. Hiochem. Hiophys. 273, 148-1...

Abstract Circulating nonadherent monocytes can migrate to extravascular sites by a process that involves adherence. Alterations in intracellular metabolism shape the immunological phenotype of phagocytes upon activation. To determine effect adherence on their metabolic and functional response human were stimulated with LPS under adherent conditions. Adherent (relative monocytes) produced less T...

Experimental evidence is presented that mitochondrial oxidative phosphorylation is dependent on oxygen concentration in its physiologic range both in vivo and in vitro. Mitochondrial oxidative phosphorylation is considered to act as a tissue oxygen sensor important for controlling local vascular resistance.

In this issue, Pospisilik et al. (2007) demonstrate that a reduction in mitochondrial oxidative phosphorylation protects mice against obesity and diabetes. This finding suggests that the moderate deficiency in oxidative phosphorylation that is observed in peripheral tissues of insulin-resistant humans is not a causative factor in diabetes but may instead be a compensatory response.

Comparative analysis of cytoplasmic organelles in a variety of tumors relative to normal tissues generally reveals a strong diminution in mitochondrial content and in oxidative phosphorylation capacity. However, little is known about what triggers these modifications and whether or not they are physiologically reversible. We hypothesized that energy substrate availability could play an importan...