The analysis of the early macrophage responses, including bacterial growth within macrophages, represents a powerful tool to characterize the virulence of clinical isolates of Mycobcaterium avium susbp. paratuberculosis (Map). The present study represents the first assessment of the intracellular behaviour in ovine monocyte-derived macrophages (MDMs) of Map isolates representing distinct genoty...

Restriction endonuclease analysis and DNA hybridization revealed five ovine strains of Mycobacterium paratuberculosis from South Africa had identical DNA patterns to an ovine strain from Canada. Genetically this strain type has features in common with the two major groups of M. paratuberculosis.

studies on the pathogenesis of babesia ovis infection following blood transfusion of infected blood to sheep with intact spleen and splenectomised sheep showed that all animals developed fever concurrent with a parasitaemia that were occurred within 2-4 days post-inoculation (dpi), clinical signs of disease were severe and included varying degrees of anorexia, listlessness, anaemia, moderate ja...

ovine pulmonary adenocarcinoma (opa) was studied in the lungs of 15 naturally-affected sheep (9 with classical and 6 atypical lesions) by transmission electron microscopy. two lung samples from normal sheep were used to develop the ultrastructural criteria. pathologic lesions consisted of focal-multifocal to coalescent nodules or masses in the cranioventral or diaphragmatic lobes. ultrastructur...

Mouse placental lactogen was purified 1840-fold from BALB/c placentae from days 14-18 of gestation with an overall yield of 29%. The purification procedure included alkaline homogenization and extraction, ammonium sulfate precipitation, hydrophobic interaction chromatography on Phenyl-Sepharose, ion-exchange chromatography on CM- and DEAE-cellulose, and gel exclusion chromatography on Sephadex ...

Natural killer (NK) cells are well recognized as playing a key role in innate immune defence through cytokine production and cytotoxic activity; additionally recent studies have identified several novel NK cell functions. The ability to study NK cells in the sheep has been restricted due to a lack of specific reagents. We report the generation of a monoclonal antibody specific for ovine NKp46, ...

BACKGROUND Endothelial cell co-culture assays are differentiation assays which simulate the formation of capillary-like tubules with the aid of a supportive cell layer. Different cell types have been employed as a supportive cell layer, including human pulmonary artery smooth muscle cells (PASMCs) and human mammary fibroblasts. However, these sources of human tissue-derived cells are limited, a...

BACKGROUND Loss of meniscal tissue is correlated with early osteoarthritis but few data exist regarding detailed biomechanical properties (e.g. viscoelastic behavior) of menisci in different species commonly used as animal models. The purpose of the current study was to biomechanically characterize bovine, ovine, and porcine menisci (each n = 6, midpart of the medial meniscus) and compare their...

Theileria annulata, a protozoan parasite of cattle and domestic buffaloes, is transmitted by ticks of the genus Hyalomma, and causes a disease named Mediterranean or tropical theileriosis. In this research 50 cattle naturally infected with Theileria annulata were treated with the extract of the plant Peganum harmala. The treatment was continued for 5 days, the dose of the extract being 5 mg/kg ...

概要 In vivoで 肺血管外水分量(pulmonary extravascular water volume, PEV)を測 定す るこ とは,1954年Chinard & Ennsに よ り示唆 されて以来,基 礎的検討がか さね られ臨床への応用が可 能 となつた.本 論文 は,Ramseyら の方法 に準 じて後天性 心疾患に おけ るPEVを 測定 し,肺 循環諸 量,臨 床経過 お よび血液 ガス値 との関連について検 討 した ものである。測定時 の肺血管内圧 とPEV とは,粗 な正相 関(r=0.48,P<0.02)を 示 すにす ぎず,む しろPEVは 左心不全の既往 と関連 が あ る とい う結果 をえた.す なわ ち測定時 の肺 血管 内圧は同 じレベ ルにあ りなが ら,左 心不 全既往のあ る例は,既 往 のない例 に比ベ てPEVは 著 明な増加を示...