نتایج جستجو برای: overlap extension pcr
تعداد نتایج: 366558 فیلتر نتایج به سال:
Here we report a PCR-based DNA engineering technique for seamless assembly of recombinant molecules from multiple components. We create cloning vector and target molecules flanked with compatible single-stranded (ss) extensions. The vector contains a cassette with two inversely oriented nicking endonuclease sites separated by restriction endonuclease site(s). The spacer sequences between the ni...
overlap syndrome, which is known as the coexistence of chronic obstructive pulmonary disease (copd) and obstructive sleep apnea (osa), was first defined by flenley. although it can refer to concomitant occurrence of any of the pulmonary diseases and osa, overlap syndrome is commonly considered as the coexistence of osa and copd. this disease has unique adverse health consequences distinct from ...
the purpose of this study was to assess the process of extension program development in iranian agricultural extension system (iaes). the research instrument was a structural questionnaire with close-ended questions which its validity and reliability was confirmed by using expert panel and cranach’s alpha test, respectively. the target population of this research included all extension managers...
A T-->C point mutation is shown to specifically inhibit PCR amplification when compared to wild-type controls in exon H of the factor IX gene. Multiple primers of different lengths and locations were designed to examine this phenomenon. The experiments suggest that poor annealing and/or extension from the downstream primer are responsible for the observed inhibition and that the mutation can ex...
During the solid-phase PCR (SP-PCR), DNA oligonucleotides complementary to a soluble template and immobilized on a surface are extended in situ. Although primarily used for pathogen detection, SP-PCR has the potential for much broader application, including disease diagnostics, genotyping, and expression studies. Current protocols for SP-PCR in microwells are suitable for enzymatic detection of...
We have established the use of a primer extension/mass spectrometry method (the PinPoint assay) for high-throughput SNP genotyping of the human Y chromosome. 118 markers were used to define 116 haplogroups and typing was organised in a hierarchical fashion. Twenty multiplex PCR/primer extension reactions were set up and each sample could be assigned to a haplogroup with only two to five of thes...
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