Osmotic cell swelling activates C l 2 channels to achieve anion efflux. In this study, we find that both the tyrosine kinase inhibitor herbimycin A and genetic knockout of p56 lck , a src -like tyrosine kinase, block regulatory volume decrease (RVD) in a human T cell line. Activation of a swelling-activated chloride current (I C l 2 swell ) by osmotic swelling in whole-cell patch-clamp experime...

A modified hypo-osmotic solution was used to select viable ejaculated and testicular spermatozoa to perform intracytoplasmic sperm injection (ICSI) in 27 treatment cycles from patients with total absence of sperm motility. The treatment cycles consisted of 15 cycles in which ejaculated spermatozoa were used and 12 cycles in which testicular spermatozoa were used. The hypo-osmotic solution consi...

The mechanisms by which fluid moves across the luminal membrane of cholangiocyte epithelia are uncertain. Previous studies suggested that aquaporin-1 (AQP1) is an important determinant of water movement in rat cholangiocytes and that cyclic AMP mediates the movement of these water channels from cytoplasm to apical membrane, thereby increasing the osmotic water permeability. To test this possibi...

The fertility potential of an artificial insemination (AI) dose is a function of the quantity, quality and health status of the semen contained therein. Therefore, identification of additional semen quality attributes associated with fertility could provide more accurate methods to predict, manage and select for AI sire fertility of predicting a bull's fertility rat. The objective of this study...

The influence of hypo-osmotic cell swelling on the activity of the mitogen-activated protein (MAP) kinases Erk-1 and Erk-2 (where Erk stands for extracellular signal-regulated protein kinase) was studied in cultured rat astrocytes. Hypo-osmotic treatment led within 10 min to an increased activity of Erk-1 and Erk-2, which became maximal at 20 min and returned to the basal level within 60 min. M...

Abstract The study was executed to investigate the different cooling periods (0, 24, and 48 hours) effects on plasma membrane integrity depending hypo osmotic swelling test (HOST), spermatozoa motility, acrosome integrity, live sperm, abnormalities, correlation among Host other rams sperm parameters This done at an animal farm in Al-Kut city (southern Baghdad city\ Iraq), from 5th January Febru...