The initiation of DNA replication in the budding yeast Saccharomyces cerevisiae occurs in two sequential and mutually exclusive steps. Prereplicative complexes (pre-RCs) containing origin recognition complex (ORC), Cdc6p, and the MCM2-7 proteins assemble only under conditions of low cyclin-dependent kinase (Cdk) activity during G(1), whereas origin activation is driven by the increase in Cdk ac...

DNA replication origins are fundamental to chromosome organization and duplication, but understanding of these elements is limited because only a small fraction of these sites have been identified in eukaryotic genomes. Origin Recognition Complex (ORC) and minichromosome maintenance (MCM) proteins form prereplicative complexes at origins of replication. Using these proteins as molecular landmar...

The six-subunit Origin Recognition Complex (ORC) is believed to be an essential eukaryotic ATPase that binds to origins of replication as a ring-shaped heterohexamer to load MCM2-7 and initiate DNA replication. We have discovered that human cell lines in culture proliferate with intact chromosomal origins of replication after disruption of both alleles of ORC2 or of the ATPase subunit, ORC1. Th...

This study addresses the question of whether native Mandarin Chinese speakers process and comprehend subject-extracted relative clauses (SRC) more readily than object-extracted relative clauses (ORC) in Mandarin Chinese. Presently, this has been a hotly debated issue, with various studies producing contrasting results. Using two eye-tracking experiments with ambiguous and unambiguous RCs, this ...

Hybrid poplar trees (Populus deltoides x nigra DN34) were grown in a green-house using hydrocarbon-contaminated soil from a phytoremediation demonstration site in Health, Ohio. Two independent experiments investigated the effect of nutrient addition on poplar growth and the importance of oxygen addition to root development and plant growth. Biomass measurements, poplar height, and leaf color we...

This paper presents the results of experimental investigation of a low-power wind energy conversion system (WECS), based on a permanent-magnet synchronous generator (PMSG) connected directly to the turbine. A development system was built in order to carry out the experiments. Its detailed description is also provided. The development system can work as an autonomous system for remote sites or a...

The Organic Rankine Cycle (ORC) has been proved a promising technique to exploit waste heat from Internal Combustion Engines (ICEs). Waste heat recovery systems have usually been designed based on engine rated working conditions, while engines often operate under part load conditions. Hence, it is quite important to analyze the off-design performance of ORC systems under different engine loads....

The sequential binding of the origin recognition complex (ORC), Cdc6p and the minichromosome maintenance proteins (MCM2-7) mediates replication competence at eukaryotic origins of DNA replication. The latent origin of Epstein-Barr virus, oriP, is a viral origin known to recruit ORC. OriP also binds EBNA1, a virally encoded protein that lacks any activity predicted to be required for replication...

Frontal EEG asymmetry patterns represent markers of individual differences in emotion reactivity and regulation, with right hemisphere activation linked with withdrawal behaviors/emotions (e.g., fear), and activation of the left hemisphere associated with approach (e.g., joy, anger). In the present study, gender was examined as a potential moderator of links between infant temperament at 5 mont...