Cryopreservation of embryos, oocytes, or ovarian tissues is the main option for female fertility preservation. Oocyte cryopreservation has emerged as especially important: the dramatic increase in the number of infants born from vitrified oocytes indicates that it is becoming one of the most important intervention options. However, oocyte cryopreservation with standard controlled ovarian hypers...

In this study, we examined the effects of reconstructed oocyte-granulosa cell complexes (OGCs) on the development of porcine oocytes derived from early antral follicles (EAFs; 0.5-0.7 mm in diameter). When denuded oocytes were cocultured with granulosa cells derived from other EAFs, the oocytes and granulosa cells aggregated to form OGCs after 2 days of culture. After 14 days of culture, we com...

BACKGROUND Laboratory animals are commonly used for evaluating the physiological properties of the mammalian ovarian follicle and the enclosed oocyte. The use of different species to determine the morphological relationship between the follicle and oocyte has led to a recognizable pattern of follicular stages, but differences in follicle size, oocyte diameter and granulosa cell proliferation ar...

In sexually reproducing animals, oocytes arrest at diplotene or diakinesis and resume meiosis (meiotic maturation) in response to hormones. Chromosome segregation errors in female meiosis I are the leading cause of human birth defects, and age-related changes in the hormonal environment of the ovary are a suggested cause. Caenorhabditis elegans is emerging as a genetic paradigm for studying hor...

objective: the measurement of follicular output rate (fort) has been proposed as a good indicator for evaluating follicular response to the exogenous recombinant folliclestimulating hormone (rfsh). this places fort as a promising qualitative marker for ovarian function. the objective of the study was to determine fort as a predictor of oocyte competence, embryo quality and clinical pregnancy af...

background: we sought to determine the association between factors that affected clinical pregnancy and live birth rates in patients who underwent in vitro fertilization (ivf) and received intracytoplasmic sperm injection (icsi) and/or laser assisted hatching (lah), or neither. materials and methods: in this retrospective cohort study, the records of women who underwent ivf with or without icsi...

The overall objective of this work was to describe whether supplementing fibroblast growth factor 2 (FGF2) during in vitro maturation (IVM) affects cumulus and oocyte maturation and subsequent embryonic development. Supplementing 0.5, 5 or 50 ng/ml FGF2 to cumulus-oocyte complexes (COCs) increased the percentage of oocytes with extruded polar bodies after IVM. Supplementing FGF2 during COC matu...

Objective This study evaluated associations of basal serum and follicular fluid (FF) anti-Muüllerian hormone (AMH) levels with in vitro fertilization (IVF) outcomes in polycystic ovary syndrome (PCOS) patients. Methods This prospective study included 179 consecutive women undergoing IVF, including 59 with PCOS and non-PCOS controls. Thirty PCOS cases had long gona-dotrophin-releasing hormone ag...

OBJECTIVE Preeclampsia (PE) occurs in 4.6% of pregnancies worldwide. The social phenomenon of increasing maternal age has raised the demand for donor oocytes. Egg donation has allowed women with poor ovarian reserve, premature ovarian failure, genetic disorders or surgical menopause to get pregnant. Recipients provide a unique model of immune response because of the differences in the genetic m...