The oligonucleotides produced by pancreatic ribonuclease and Taka-Diastase ribonuclease TI digestion of 32P-labeled ribosomal 5 S RNA derived from KB cells (human epidermoid carcinoma line) have been isolated and their nucleotide sequences determined. The oligonucleotides can all be separated from one another by two-dimensional electrophoresis. The position of the oligonucleotides was ascertain...

The complexation of oligonucleotides and antisense drugs with a cationic surfactant, cetylpyridinium chloride (CPC), has been studied using electrochemistry at a micrometer sized liquid/liquid interface. This method can be used to investigate the effect of chemical and structural modifications on the complexation behaviour of oligonucleotides. For the interaction between CPC and oligonucleotide...

Synthetic oligonucleotides are the main cost factor for studies in DNA nanotechnology, genetics and synthetic biology, which all require thousands of these at high quality. Inexpensive chip-synthesized oligonucleotide libraries can contain hundreds of thousands of distinct sequences, however only at sub-femtomole quantities per strand. Here we present a selective oligonucleotide amplification m...

Programmed ribosomal frameshifting is a translational recoding mechanism commonly used by RNA viruses to express two or more proteins from a single mRNA at a fixed ratio. An essential element in this process is the presence of an RNA secondary structure, such as a pseudoknot or a hairpin, located downstream of the slippery sequence. Here, we have tested the efficiency of RNA oligonucleotides an...

Chemically synthesized combinatorial libraries of unmodified or modified nucleic acids have not previously been used in methods to rapidly select oligonucleotides binding to target biomolecules such as proteins. Phosphorothioate oligonucleotides (S-ODNs) or phosphorodithioate oligonucleotides (S2-ODNs) with sulfurs replacing one or both of the non-bridging phosphate oxygens bind to proteins mor...

The solid phase synthesis of oligonucleotides at smaller scales is well understood and the processes at this scale do not impose any impact on economic, environmental or safety considerations. However, apart from economic consideration, significant challenges still exist for large-scale manufacture. More than a decade’s effort and continuing searches for therapeutic agents based on antisense, r...

Antisense oligonucleotides have been used for a number of years to modify the expression of specific genes both in vivo and in vitro (Scanlon et al., 1995). The most potent mode of antisense activity is through RNase H–mediated degradation of RNA (Figure 1). The RNase H– endonuclease specifically cleaves RNA only when it is hybridized as a heteroduplex with DNA. In general, oligonucleotides tha...

To test further the idea that sexual behavior in rodents is mediated via the progesterone receptor (PR) in the ventromedial nucleus of the hypothalamus, antisense and sense oligonucleotides to progesterone receptor were administered intracerebroventricularly into the third cerebral ventricle of ovariectomized estrogen-primed animals. Progesterone-facilitated sexual behavior was inhibited in ani...