In the present investigation 28 genotypes of soybean were analysed using 37 start codon targeted (SCoT) markers and primers produced 260 DNA fragments with an average 7.03 bands per primer. From these primers, SCoT 33 65 wos most polymorphic, where 10 polymorphic amplification products detected. The lowest number amplified (2) was detected by 8, 14, 19, 31 59. Of bands, 200 (77.27 %) 5.41 To de...

There are a number of applications of molecular markers in agriculture such as assessing genetic diversity, generating DNA fingerprints and developing markers linked to a trait of interest, etc. Arbitrarily Primed-Polymerase Chain Reaction (AP-PCR) in which template DNA is amplified using single arbitrary primers of 10 base in length is a widely used technique. The objectives of this investigat...

The genetic diversity of 20 Iranian populations of Bunium persicum has been evaluated with random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers. Fresh leaves of seedlings from each population were used for genomic DNA extraction. Analysis of banding patterns of 15 RAPD primers and 17 AFLP primer combinations, revealed 192 (86%) and 228 (75%) polymorp...

This study was performed out genetic diversity of some Elaeagnus angustifolia L. populations growing in İzmir province by using ISSR markers. In the study, PCR 15 primers. products were run agarose gel and visualized under UV light. Amplified scored as follows. A total 46 bands produced from primers, which 27 polymorphic. The proportion polymorphic evaluated approximately 58.7%. Genetic distanc...

ustilago hordei (pres) lagerh is a fungus that causes covered smut of barley. this disease reduces yield and is a limiting factor in barley fields of iran. one hundred forty four isolates of u. hordei were collected from 36 regions of khuzestan province during 2009 and the genetic diversity of 100 isolates was evaluated by molecular markers. four out of 14 primers were selected on the basis of ...

In this study, we report a simple procedure for developing and using new types of polymerase chain reaction (PCR) primers, named ‘‘high-frequency oligonucleotides–targeting active genes’’ (HFO-TAG). The HFOTAG primers were constructed by first using a ‘‘practical extraction and report language’’ script to identify oligonucleotides (8, 9, and 10 bases) that exist in high frequency in 4700 expres...

The genomic DNA polymorphism and phytochemical variation of Stevia rebaudiana Bertoni was investigated by RAPD-PCR and HPLC analysis. For initial PCR screening, about hundred oligo-nucleotide primers were used to amplify the genomic DNA from three accessions of S. rebaudiana (L1 to L3). The concentration of stevioside content was quantified by using HPLC analysis. A total of 9 primers were fina...

Fifteen serotypes of E. coli isolated from poultry were subjected to RAPD-PCR using eight oligonucleotide random primers. Amplification with each of the primers resulted in generation of different DNA fingerprinting profile with varied number of bands. Dendrogram based generation of clustering of E. coli serotypes showed two major clusters. The polymorphic amplicons served as markers for E. col...

First line in abstract should be Date palm is an important fruit crop of the arid and semiarid region. In the present investigation two types of DNA based marker RAPD and ISSR were used to characterize 8 date palm genotypes grown in the Kutch region. Amplification of genomic DNA of 8 genotypes using 13 RAPD analyses yielded 88 fragments, of which 35 were polymorphic, with an average of 2.69 pol...