Thiodiglycolic acid has been identified as a major metabolite of the anticancer drug ifosfamide in humans. Patients treated with 12-16 g ifosfamide/m2.day excreted thiodiglycolic acid ranging from 0.10 +/- 0.02 mmol on the first day of therapy, to a maximum of 3.27 +/- 0.15 mmol on the fourth day of ifosfamide infusion. This amounted to 5.4 +/- 0.2% of the administered dose of ifosfamide appear...

Thiodiglycolic acid has been identified as a major metabolite of the anticancer drug ifosfamide in humans. Patients treated with 12–16 g ifosfamide/mzday excreted thiodiglycolic acid ranging from 0.10 6 0.02 mmol on the first day of therapy, to a maximum of 3.27 6 0.15 mmol on the fourth day of ifosfamide infusion. This amounted to 5.4 6 0.2% of the administered dose of ifosfamide appearing as ...

The binding interactions between ligands and receptors play a vital role in their biological functions. In this work, a label-free indirect competitive binding assay based on surface plasmon resonance (SPR) detectionwas developed, using fatty acid–human serumalbumin (HSA) as amodel system. In the assay, a fatty acid analog was immobilized on the surface of an SPR gold chip by self-assembly, and...

The ability of the fatty acid binding sites of serum albumin to bind lipoteichoic acid of Streptococcus pyogenes was investigated. Initial studies indicated that lipoteichoic acid, but not its deacylated deprivative, protected albumin from being denatured by heat (80C for 1 h) and changed its mobility in an electrical field. Albumin covalently linked to agarose beads bound radiolabeled lipoteic...