To produce Aspergillus oryzae alkaline protease (Alp) in an osmophilic yeast Zygosaccharomyces rouxii, we constructed an expression plasmid consisting of the Z. rouxii glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter, the prepro-Alp cDNA of A. oryzae, the whole sequence of Z. rouxii plasmid pSR1, and the G418 resistant gene. The resulting plasmid, when introduced into Z. rouxii cells, ...

A manganese peroxidase gene (mnp1) from Phanerochaete chrysosporium was efficiently expressed in Aspergillus oryzae. Expression was achieved by fusing the mature cDNA of mnp1 with the A. oryzae Taka amylase promoter and secretion signal. The 3' untranslated region of the glucoamylase gene of Aspergillus awamori provided the terminator. The recombinant protein (rMnP) was secreted in an active fo...

The rice blast fungus, Magnaporthe oryzae, is a model plant pathogenic fungus and is a severe threat to global rice production. Over the past two decades, it has been found that the peroxisomes play indispensable roles during M. oryzae infection. Given the importance of the peroxisomes for virulence, we review recent advances of the peroxisomes roles during M. oryzae infection processes. We fir...

In the present study, mould mycelium of Aspergillus oryzae was used for biochemical conversion of L-tyrosine to L-DOPA because tyrosinase is an intracellular enzyme. The mutant Aspergillus oryzae IIB-6 was found to yield 3.72 fold higher production of L-DOPA than the parental strain. The comparison of kinetic parameters was also done which showed the greater ability of mutant to yield L-DOPA. W...

Nigrospora oryzae an endophytic fungus isolated from the leaf of a climber Pterolobium hexapetalum (Roth.) Sant. and Wagh. produced bioactive compound which are active against human pathogens. The organic extract (ethanol) of the endophyte Nigrospora oryzae was separated using HPLC and tested for biological activities. The ethanol extract was tested for antibacterial and anti fungal activity us...

of a Dissertation Su bmitt ed i n partial fulfilment of t he requirements fo r t he degree of Doctor of Philosophy i30 STON UNI VEI10ITY GllADU AT,t; .SCHOOL

ω-Transaminases display complicated inhibitions by ketone products and both enantiomers of amine substrates. Here, we report the first example of ω-transaminase devoid of such inhibitions. Owing to the lack of enzyme inhibitions, the ω-transaminase from Ochrobactrum anthropi enabled efficient kinetic resolution of α-methylbenzylamine (500 mM) even without product removal.

BACKGROUND Symbioses between metazoans and microbes are widespread and vital to many ecosystems. Recent work with several nematode species has suggested that strong associations with microbial symbionts may also be common among members of this phylu. In this work we explore possible symbiosis between bacteria and the free living soil bacteriovorous nematode Acrobeloides maximus. METHODOLOGY W...