Miner1 is a redox-active 2Fe2S cluster protein. Mutations in Miner1 result in Wolfram Syndrome, a metabolic disease associated with diabetes, blindness, deafness, and a shortened lifespan. Embryonic fibroblasts from Miner1(-/-) mice displayed ER stress and showed hallmarks of the unfolded protein response. In addition, loss of Miner1 caused a depletion of ER Ca(2+) stores, a dramatic increase i...

Quantitative mapping of metal ions freely diffusing in solution is important across a diverse range of disciplines and is particularly significant for dissolution processes in batteries, metal corrosion, and electroplating/polishing of manufactured components. However, most current techniques are invasive, requiring sample extraction, insertion of an electrode, application of an electric potent...

Alterations in the synthesis and degradation of proteins were investigated in intact lungs exposed to the volatile anaesthetic halothane. In rat lungs perfused in situ with Krebs-Henseleit bicarbonate buffer containing 4.5% (w/v) bovine serum albumin, 5.6 mM-glucose, plasma concentrations of 19 amino acids and 690 microM-[U-14C]-phenylalanine and equilibrated with O2/N2/CO2 (4:15:1), protein sy...

Although lung diffusing capacity for carbon monoxide (DL(CO)) is a widely used test of diffusive O2 transfer, few studies have directly related DL(CO) to O2-diffusing capacity (DL(O2)); none has used the components of Dl(CO), i.e., conductance of alveolar membrane and capillary blood, to predict DL(O2) from rest to exercise. To understand the relationship between DL(CO) and DL(O2) at matched le...

Heart rate during overnight rest and while diving were recorded from five emperor penguins with a microprocessor-controlled submersible recorder. Heart rate, cardiac output and stroke volume were also measured in two resting emperor penguins using standard electrocardiography and thermodilution measurements. Swim velocities from eight birds were obtained with the submersible recorder. The resti...

In this paper C denotes a category and o1, o2, o3 denote objects of C. Let C be a non empty category structure with units and let o be an object of C. Observe that 〈o, o〉 is non empty. The following propositions are true: (1) Let v be a morphism from o1 to o2, u be a morphism from o1 to o3, and f be a morphism from o2 to o3. If u = f · v and f −1 · f = id(o2) and 〈o1, o2〉 6= ∅ and 〈o2, o3〉 6= ∅...

A reaction involving the catalytic oxidation of glucose by the enzyme glucose oxidase is employed as a first spatially resolved experimental realization of a nonclassical A + C f C + Products chemical reaction. This heterogeneous kinetics experiment models the operation of optical glucose sensors. One-dimensional nonclassical reaction kinetics, manifested in anomalous rate laws, are observed. T...

Mechanisms that control the levels and activities of reactive oxygen species (ROS) in normal human mammary cells are poorly understood. We show that purified normal human basal mammary epithelial cells maintain low levels of ROS primarily by a glutathione-dependent but inefficient antioxidant mechanism that uses mitochondrial glutathione peroxidase 2. In contrast, the matching purified luminal ...

in order to study of effects of deficit irrigation on morphological traits and growth indices variation of corn forage (sc. 704) in rasht region, an experiment based on randomized complete design with four treatments and three replication was conducted in 2007 growing season, at research farm of faculty of agriculture, university of guilan. experimental treatments consisted of: no-irrigation, 1...